Enzymes Question

[fox914]

What two things can cause enzymes to malfunction?

I couldn't find the answer in my texbook.

[James]

Maybe pH, temperature etc

[kotoreru]

Incorrect folding of the protein structure, inappropriate di-sulphur bridges, having half missing...

[victor]

There're many factors that can cause enzyme malfunction. These factors can start to happen even in the earliest stage of enzyme production which is transcription, which we call it along with the translation as a pre-translational phase. After that, the fault can be happer also in the post-translational modification such as folding and disulfide bond establishment. Both the pre and the post translational thng is called as intrinsic factors.
While extrinsic factors could be temperature, pH, activator, and inhibitor...

[soso]

search about enzyme denaturation you will understand..

[MrMistery]

Also inhibitors...

[kanak33]

Yes, all are right.
Heat, pH, inhibitors can cause malfunctions.
But the temperature denatures the enzymes and its irreversible (right I hope )

Nope heat inactivation is not always irreversible. In many case simple proteins simply renature when the temperature goes down.

[Final_King]

heat and ph do causes malfunctions or really it s not but it is denature if denaturing counts. but only after certain tempertures when they get too high at least the ones my classs did " they tend to denature the enzyme or i guessmake them malfuntion didnt get to see what was going on wiht enzymes so thats up for grabs if someone can explain that.", anyway they the ph and temps just increases the enzyme activity.

[Duke]

What two things can cause enzymes to malfunction?

I couldn't find the answer in my texbook.

If malfunction means not the function which it should have and not loss of function, then I have 2 examples for you:

Solvent can inlfuence the substrate specificity of some enzymes (Restriction enzymes sometimes show star activity when used with inappropriate buffers)

Co-factors like Mg or Mn can also determine the action of enzymes (e.g. DNaseI cleaves DNA in the presents of Mg in a random manner (each strand independently) but in the presents of Mn it generates blunt ends)