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to get the sequence of the promoterModerator: BioTeam
3 posts • Page 1 of 1
to get the sequence of the promoterHi,
I'd like to analyse the promoter sequence (let's say 1kbp) of a gene using Transfac. But first, I need the sequence... Does anyone know how to get the sequence located in the 5' of the first exon on a gene of interest ? any suggestion very welcome, snoopline
Ok I got it
Go on http://www.dsi.univ-paris5.fr/genatlas find your gene of interest by filling the form (symbol name or id OMIM). on the result page click on the gene you are interested about a page with a lot of information is displayed In the DNA paragraph you'll find a link : 10 Kb 5' upstream gene genomic sequence study Just click on it to get the sequence It's a little bit to long for alibaba2 so cut it and paste it in alibaba2 If others find other solutions, feel free to share. Snoopline
Re: to get the sequence of the promoterA great site http://www.mybioinfo.info , where you can search your genes using symbol, NCBI RefSeq or words.
And, 5' up-stream 10 kb promoter region is right there for you. It tells you which strand the gene is on. And 10 kb is in the 5' up-stream for sure. See the number yourself. This site lists everything, mRNA, protein and genomic sequences in a tabular format. You even can add genes to your gene list and download multiFASTA format of sequences. Neat!
3 posts • Page 1 of 1
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