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The effect of temperature on the survival of yeast cells

Debate and discussion of any biological questions not pertaining to a particular topic.

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Postby spoongirl » Sat Apr 28, 2007 3:01 pm

the actual temperature that kills all of the yeast cells is 66.65 °C
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Postby Ulbrid » Sat Apr 28, 2007 3:10 pm

sarah briggs wrote:Does any one know why the Enzymes from the respiring yeast cells cause Meth Blue to become colourelss (the actual scienific reasoning), My teacher hinted that ions where involved??
Thanks


Heh, no; all my teacher told me was that it turns colourless when there's enzyme activity- beyond that, i really haven't a clue. I don't think it really matters, anyway.
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Postby yagetme » Sat Apr 28, 2007 5:20 pm

hi all! in the cswk to do with the yeast cell, can any1 help me with the preliminary section, i really dnt know wht to write thanks
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Postby yagetme » Sat Apr 28, 2007 7:07 pm

answer me sum 1 plese im soooo stck
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Postby 01addiv-llangirb » Sat Apr 28, 2007 7:43 pm

Hi,

Did you do any kind of prelim?
Physics more and more knowledge about less and less. - Still it's the basis for all else.
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Postby kjit » Sun Apr 29, 2007 12:36 pm

what do we include in the scientific explanation

cant you just add both solutions, then heat up the test tubes?? then add methylene blue.... nd examine under microscope :?
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Re: The effect of temperature on the survival of yeast cells

Postby kjit » Sun Apr 29, 2007 12:38 pm

what do we include in the scientific explanation

cant you just add both solutions, then heat up the test tubes?? then add methylene blue.... nd examine under microscope :?
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Postby 01addiv-llangirb » Sun Apr 29, 2007 3:18 pm

Kjit thats it exactly it only needs to be that simple. Explenation is tertiary structure of enzymes thats all. good luck
Physics more and more knowledge about less and less. - Still it's the basis for all else.
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effects of temperature on yeast

Postby Katewoo » Sun Apr 29, 2007 3:49 pm

To be honest i used a colorimeter once and am pretty sure i broke it. unfortunetly it seems that we're meant to use it, but i haven't got a clue how you use it atall? Do we have to describe in full how we use it? or just that state that we did use it?and if we do have to describe in full could someone please tell me how. thank you x
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Postby 01addiv-llangirb » Sun Apr 29, 2007 3:58 pm

my advice would be don't bother the solution is to opaque for it to work well - count the cells under a microscope. Sorry if your set on using one i am sure someone will work out how - i jsut dislike them.
Physics more and more knowledge about less and less. - Still it's the basis for all else.
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Postby wannabeaditz » Sun Apr 29, 2007 5:52 pm

i'm using a colorimeter as it is pretty easy to use
make a blank with no meth blue put in cuvette press R. then one by one add the heated experimented on samples and press T. you get the % transmission of light. This can then be drawn on a graph really easily. The only bad thing is that they don't measure exactly below one. If the number 1 is flashing it means below one.
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Postby krosey13 » Sun Apr 29, 2007 8:21 pm

a colorimeter has to be used to make the plan of alevel standard otherwise it would be like GCSE cwk...
and the seperate solutiond have to be heated up to a constant temp befor they can be mixed together so that it makes a fair test, otherwise all substantces wont be of the same temp.
and in theory part u have to say how u need a pH buffer of 7-7.4 to maintain the pH and keep it constant.
And how subtrate-complexes are less likely in a temp above optimum and eventually stop. cos 3-d active site looses its 3-d ness
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