Discussion of all aspects of cellular structure, physiology and communication.
First of all, would that be 'foo' for the legendary band that is the foo fighters? if yes, there my fave band so woohoo!
Um, microscope or colorimeter? Well the colorimeter will tell you the exact amount of light that is being transmitted through the solution (100% would be a perfectly clear solution so the cells would all be alive, 40%ish would mean they were all dead [ive been told]) but if you have a good eye for colours a microscope could be ok, but a colorimeter is more accurate. Got any tips of your own?
Thank you for that, i'll go add that now!
The problem is: the solution won't be perfectly clear since the yeast suspension is cloudy. In fact, it would be difficult to get any kind of reading because i doubt any light will be transmitted through the solution (unless heavily, and accurately, diluted).
Therefore, i think using a microscope is more accurate because you can count the individual blue and colourless yeast cells and calculate percentages of living/dead cells.
I'm a little bit confused I've written all about enzymes in my background info part and I've just reallised I haven't mentioned yeast, where so enzymes come in to the fermentation of yeast? I know this is a stupid question but I've just confused myself thoroughly!
and it says that kills all yeast cells, yeast is a fungi not an enzyme so what has the enzymes being denatured got to do with the yeast cells dying? Anyb help on this would be greatly appriciated.
Also how accurate do we have to be with the temperature? I've done every 5 degrees and said in my limitatioss that id I had more time I would have done 1 degree increments, is that alright do you think?
If you destroy the enzymes the organism needs to survive, have you not effectively "killed" them.
The accuracy of the result should be limited by how fine a temperature grid you sample; the finer the grid, the more accurate the result, in principle.
one of those haemocytometer slides is what my teacher said to use and then calculate the percentage that are dead or alive (dead would be blue, alive would be clear). Would a colorimeter work because the liquid would still have the yeast solution in and so would never be actually clear. or am i just having a blonde monent??
And also, I'm not named after the Foo Fighters, it's just what a handful of my friends call me. how odd. but i am also a fan of said band so yay!
Yeah I tried using a colorimeter in my prelims and it didm't work because the solution was too cloudy so I said that in my plan I think the haemocytometer is the best option by far because with a normal microscope slide it is difficult to count the same size area for each sample.
Does anyone know if there is one of these forums for the A2 practical as I'm doing them both at the same time and the A2 one is HARD! x
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