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effect of temperature on the survival of yeast cells

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Postby 01addiv-llangirb » Thu Apr 26, 2007 7:20 pm

Having done this experiment in full today haha don't you love ocr

The best way of using the indicator i found was to ferment the yeast a bit at whatever temp then shake and using a teat pipette remove a little place 1 drop on a slid then add 1 drop of indicator place a corev slip on and examine the slid under a microscope 400x magnification is enough. cells that are clear (they are round) are alive if they are blue they are dead count how many of each in one veiw and then work a percentage out esay peasy. :)
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Postby CrimsonFalcon » Thu Apr 26, 2007 7:25 pm

that seems like a very annoying way of doing it, but i bet it was fairly accurate. the only problem is with that, you have to go into how it could give incorrect results through your random samlping as you cant really count every dead cell, certainly not easily!
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Postby 01addiv-llangirb » Thu Apr 26, 2007 7:28 pm

But it is far far easier then dying the entire solution and then trying to distinguish between live and dead
Physics more and more knowledge about less and less. - Still it's the basis for all else.
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Postby CrimsonFalcon » Thu Apr 26, 2007 7:30 pm

yeah, that makes perfect sense.

theres only one thing im not sure about now really... do i put the equipement as a list? like bullet points or, in one big paragraph explaining the use of each thing which you can guess from the plan kinda anyway?
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Postby 01addiv-llangirb » Thu Apr 26, 2007 7:32 pm

generally i always make a bullet point list it has always got me the marks for that bit and keeps the word count down - i have problems with keeping it that short.
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Postby CrimsonFalcon » Thu Apr 26, 2007 7:36 pm

im not having probelms with the work limit, maybe because im not sure if i need to write a theory/ what to put into it .. :/

yeah. in bio a list seems like a better idea. i cant remmeber what subject it was but they said write it as a para, but come to think of it, not so much in Bio. thanks alot there

.../falling asleep on chair xD
at least this is 'apparently' easier than the yr. 13 one or so i have heard hehe. ill be gratefull to that.
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Postby 01addiv-llangirb » Thu Apr 26, 2007 7:40 pm

You need to put a theroy/hypothosis in ie at 50 most are dead for example

The second year one is i belive on Photosynthisis and invloves four seperate experiments - aparently a right pig.

Where abouts are you doing this?
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Postby CrimsonFalcon » Thu Apr 26, 2007 7:43 pm

well, ive said i think it will die liek around 55-65 roughly, and said thats cause i fround out on an internet site that it dies at 58 degrees, but they are not always correct on the internet so ive taken a rough amount to be safe.

im workin from home at the mo... its just im tired now hehee *dies*

Gl to all who are doing this ^_^
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Postby 01addiv-llangirb » Thu Apr 26, 2007 7:49 pm

Yeah all yeasts are slightly different thats part of the great thing about biology - very few constants.

I ment where abouts in the country.

Thanks Alot for that temperature data a really big help (saves me looking)lol
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Postby CrimsonFalcon » Thu Apr 26, 2007 7:50 pm

hey.. heres something ammusing i found out on methylene... *grins*

put a few drops in someones coffee and thier urine will turn green, as a prank (or more blue-ish colour the more you put in) you cant taste it much nither, but allergie affects acn be caused (thats something you can put into safety - dont eat it!)
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Postby CrimsonFalcon » Thu Apr 26, 2007 7:53 pm

your welcome with that ^_^ hehe. well, umm England. same as ju
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Postby 01addiv-llangirb » Thu Apr 26, 2007 7:54 pm

excellent still beats the last plan i wrote we used phenophaline so under saftey i put: "its an elephant laxative the risks are obvious!"

Don't forget under saftey some people have allergic reactions to yeast in 'live' format.

Do you know which of the 5 enzymes in yeast the methylene... is reacting with?
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