Discussion of all aspects of cellular structure, physiology and communication.
The methylene is the indicator, you put a few drops into the yeast before you add the sugar solution. You don't have to leave the yeast and the methylene for a certain time, just mix them together. In my plan I add the sugar soulution to the yeast then leave it for 5mins to see if the colour changes. Also, the only way to get a graph of your results is to use a colourimeter, which measures how much light passes through a sample of liquid.
Anyway, you mix the methylene with the yeast, you don't have to leave the two together for any period of time
I'm considering using a haemocytometer and microscope to physically count the number of yeast cells remaining stained at each selected temperature and using with the results plot a graph and use a line of best fit. But i'm concerned that the yeast's enzyme for anaerobic respiration should all cease to work at the same temperature. Using a colorimeter wouldn't you get the same sort of problem i.e no gradual colour change?
Any ideas on this?
Hi i've just been given this too I;m probably going to leave it at the most 5 minutes.
What have you done for your prediction?
I really need your help!!!
you get cold yeast suspension....add a few drops of the methylene blue.....mix it together, it's now blue. You put this mixture into a water bath at whatever temperature you want it at for about 10mins. You then take out the yeast suspension. Then you add the sugar solution to the yeast and see if the blue colour changes. If the blue colour disappears or starts to disappear, there are still yeast cells that haven't been denatured. Is this clear?? If the blue stays as it is, the enzymes are not functioning, which means they have been denatured by the heat.
There seems to be lots of confusion surrounding the methylene blue. It is an indicator, you only add a few drops of it. You mix it into the yeast, you don't add them together and then leave them for 5 mins, you mix them. Add it before you heat the yeast, otherwise it will cool the yeast down.
DOES EVERYONE UNDERSTAND?!!!
to the person who wanted to use the microscope, wouldnt that mean that you didn't need to use glucose at all, because if you use a microscope to see which cells have been stained won't the glucose get in the way?? im very confused about that because that is what i wanted to do
You need a sugar to see whether the yeast is respiring. I very much doubt you'd see a molecule as small as glucose under a light microscope.
Has anyone found roughly the temperature that the enzymes for respiration IN YEAST becomes denatured
could you dilute the solution before u add it to microscope slide so that the cells are easier to count rather than using a haemocytometer?
yes it denatures around 70 degrees, but thats when i used the methylene blue in the solution. It didnt turn completely colourless.
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