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Histology Help

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Histology Help

Postby radical1080 » Sat Mar 17, 2007 9:31 am

Hiya, having a little (well actually alot) of trouble identifying this slide image. Im a second year biomedical science student and no one has yet been able to identify it out of a class of 200 or so (if someone has they're keeping it well hidden). Any help at all would be much appreciated. Cheers.

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Postby Darby » Sat Mar 17, 2007 4:37 pm

Any clues at all, such as the magnification? From your class description, it's human tissue, right-? And is it safe to assume it's not some sort of pathology? (It actually looks like a nematode lamina, but it wouldn't be that...)

Some observations -

- Looks like one of the basic blue stains, which should stain nuclei but are otherwise fairly uniform.

- It doesn't look cellular (without knowing the power, that may not mean anything), which suggests connective tissue matrix (which also is commonly stained with blue stains that will color the proteins). It's not dense, though, which probably rules out cartilage, tendon, ligament, and bone (except for some marrows). That suggests one of the fibrous varieties. That's all I've got.

I TA'ed histology for a few years, and one of the other TA's loved to set up slides in weird ways to stump the lecture professor. Some things are hard to identify no matter how much experience you have.
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Postby Dr.Stein » Sun Mar 18, 2007 2:05 am

We need some data to be able to figure it out. Plant or animal tissue? It seems like sugarcane epidermis, but it also seems like fenestrate membrane in artery or something squamous epithelium hmm.. :?
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Postby radical1080 » Sun Mar 18, 2007 9:52 am

The magnification is unknown and it should be a human tissue sample. The only thing given was this slide.
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Postby Vagabond » Mon Mar 19, 2007 2:42 pm

That is tough,
off the cuff, it looks like it could be a really poorly stained cornea or perhaps the outer layer of skin (Stratum Corneum). But I am not sure of that I will think a little bit more on it.
Last edited by Vagabond on Tue Mar 27, 2007 2:56 pm, edited 1 time in total.
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Postby Caspase » Tue Mar 20, 2007 9:47 pm

My first guess was mitocondrial christae but em is not that blue.

Could be an artery because those could retic fibers.

Final answer that im gonna stick with is longitudinal nerve section with the arrow pointing to a node of ranvier.
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Postby Caspase » Mon Mar 26, 2007 1:23 pm

What was the sample of???????????
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