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How old is the ancestral protein likely to be.

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How old is the ancestral protein likely to be.

Postby go&go » Wed Mar 07, 2007 2:26 am

hi, anyone could shed some light on this puzzle pls,
how to estimate when in evolution did a ncestral protein arise, in other words, How old is the ancestral protein likely to be? detail as bellow,

there is a amino-acid sequence of part of the AntP gene product , KRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHAL

Use this to do a BLAST search to find other proteins related to Drosophila AntP. First open the BLAST page at NCBI. Choose the Standard Protein Blast option (BlastP), and copy and paste the sequence into the search box. Click "Blast!" to do the search, then click the Format button to see the results. This will give a long list of proteins that match the sequence, either approximately or exactly. How old is the ancestral protein likely to be.

i v got the long list of protiens and the distance tree, however , i can tell how old it is . thanks
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Postby mith » Wed Mar 07, 2007 3:19 am

You're probably going to need to find a probabilty of how much the protein mutates over time, and then you need to find some sort of reference protein whose age we already know.
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Postby go&go » Wed Mar 07, 2007 4:11 am

mith wrote:find a probabilty of how much the protein mutates over time, and then you need to find some sort of reference protein whose age we already know.


but the list of protien i found does not make sense , that as bellow:

Sequences producing significant alignments:.....................................Score(Bits)
gi|9837164|gb|AAG00456.1|AF277988_1 LIN-39 [Caenorhabditis br... 58.2 1e-07
gi|47228654|emb|CAG07386.1| unnamed protein product [Tetraodon n 58.2 1e-07
gi|37029992|gb|AAQ88098.1| hox protein Dfd [Schistosoma mansoni] 58.2 1e-07
gi|2209198|gb|AAB61441.1| LOX6 [Helobdella robusta] 57.8 1e-07
gi|72008704|ref|XP_780219.1| PREDICTED: similar to homeobox [Str 57.8 1e-07
gi|74267559|dbj|BAE44278.1| hoxC4a [Oryzias latipes] >gi|8301... 57.8 1e-07
gi|86560752|gb|ABD04657.1| Hox7 homeobox protein [Nereis virens] 57.8 2e-07
gi|108869675|gb|EAT33900.1| conserved hypothetical protein [Aede 57.8 2e-07
gi|1574928|gb|AAB09405.1| HEHBOX6 57.8 2e-07
gi|119370800|sp|Q1KKR6|HXD4B_FUGRU Homeobox protein Hox-D4b >... 57.8 2e-07
gi|396779|emb|CAA52159.1| sex combs reduced homeodomain prote... 57.8 2e-07
gi|18858849|ref|NP_571219.1| homeo box C5a [Danio rerio] >gi|... 57.8 2e-07
gi|123295378|emb|CAM16416.1| homeo box C5a [Danio rerio] 57.4 2e-07
gi|120974711|gb|ABM46720.1| HOXD8 [Gorilla gorilla] 57.4 2e-07
gi|74096243|ref|NP_001027781.1| hox4 protein [Ciona intestina... 57.4 2e-07
gi|306879|gb|AAA36007.1| homeo box c8 protein 57.0 3e-07
gi|24497544|ref|NP_710160.1| homeobox C6 isoform 2 [Homo sapi... 57.0 3e-07
gi|51474|emb|CAA34517.1| unnamed protein product [Mus musculus] 57.0 3e-07
gi|18026216|gb|AAL25809.1| antennapedia homeodomain protein [Eup 57.0 3e-07
gi|66476112|gb|AAX63763.2| HoxC6bii [Oncorhynchus mykiss] 57.0 3e-07
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