Experiments with S. aureus on Agar

[saternet]

I am conducting an experiment to grow Staphylococcus Aureus standard nutrient agar medium; I've incubated for THREE days and seen no growth (at 20 C). I am now incubating the same samples at around 32-37C and for about 14 hours, I am seeing no growth. The colour of my agar is slightly yellow. I swabbed agar with a standard q-tip and sterile qtip; should I stop the experiment??

[Caspase]

Try 37 C anything less and you wont get good results. After 48 hrs you should have a considerable amount of colonies.

Are you using the correct nutrients in your agar?

[saternet]

Alright then thanks; I am incubating the same dishes at 37 C and I will do it for 48 hours

I am using nutrient agar with beef extract and yeast; just the standard one I mixed in the lab.

Its been about 8 hours since I wrote the last post so I am 22 hours into my incubation. I am also seeing that there is a load of condensation on the petri lids, water is just sloshing around.

[saternet]

your advice helped a lot. I am spotting some colonies within the first 35 hrs. thanks!