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Fig. 1. The 50%-majority-rule consensus trees of the S, M, and L segments. Trees were constructed in mrbayes by using the general time-reversible model, with γ distributed rate variation across sites. Posterior probabilities are indicated above each branch. The nomenclature of the isolates is as follows: state of origin, collector, and order of sample isolation; clones taken from a single clover are identified with an alphabetical suffix that is serially assigned. Gray shading indicates phylogenetic grouping based on clades within the small segment; previously isolated Cystoviridae are two-tone. The scale bar indicates 0.1 substitutions per site.
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Fig. 2. Distributions of tree similarity metrics. (A) Number of resolved, shared identical triplets. Bars indicate the random expectation of identical triplets for 20,000 tree-to-tree comparisons. Arrows indicate the median number of identical triplets found between segment phylogenies, none of which showed statistical significance (see text). (B) Number of resolved different triplets. Bars indicate the random expectation of different triplets for 25,000 tree-to-tree comparisons. Arrows indicate the median number of different triplets between 500 segment phylogenies (25,000 comparisons); only the median S-M segment comparison was found to be statistically significant (see text).
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