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In this study, the authors present evidence showing that while SERPINI1 is …


Biology Articles » Molecular Biology » Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner » Table

Table
- Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner

Table 1
Primer sequences used to amplify intergenic fragments for subsequent cloning
Construct*
Primer sequence

(Forward)
(Reverse)
pGL3-Basic constructs
1. SERPINI1 (1-851)
5'-ATAGATCTACTCCGGCGACGCCGGA-3'
5'-ATAAGCTTGTCCAGACTGCGCCTCT-3'
2. SERPINI1 (1-175)
5'-ATAGATCTACTCCGGCGACGCCGGA-3'
5'-ATAAGCTTTCGCCTCAGGATCCCCC-3'
3. SERPINI1 (176-851)
5'-CGAGATCTTGGGTGGTGACACTTCT-3'
5'-ATAAGCTTGTCCAGACTGCGCCTCT-3'
4. SERPINI1 (474-851)
5'-AGAGATCTGTGTGGGGACTTGCACT-3'
5'-ATAAGCTTGTCCAGACTGCGCCTCT-3'
5. SERPINI1 (711-851)
5'-ATAGATCTCAGGGCAGCTGCTTTCC-3'
5'-ATAAGCTTGTCCAGACTGCGCCTCT-3'
6. PDCD10 (851-1)
5'-ATAGATCTGTCCAGACTGCGCCTCT-3'
5'-ATAAGCTTACTCCGGCGACGCCGGA-3'
7. PDCD10 (710-1)
5'-TCAGATCTGTACCTTTCCGCTCCCT-3'
5'-ATAAGCTTACTCCGGCGACGCCGGA-3'
8. PDCD10 (473-1)
5'-ATAGATCTCAAGCCCTCCCTTCACC-3'
5'-ATAAGCTTACTCCGGCGACGCCGGA-3'
9. PDCD10 (175-1)
5'-ATAGATCTTCGCCTCAGGATCCCCC-3
5'-ATAAGCTTACTCCGGCGACGCCGGA-3'
10. PDCD10 (107-1)
5'-ATAGATCTGCCGGCGAGCGGGACTA-3'
5'-ATAAGCTTACTCCGGCGACGCCGGA-3'
11. PDCD10 (175-108)
5'-ATAGATCTTCGCCTCAGGATCCCCC-3'
5'-CCAAGCTTGTTGTCCGCAATTCCTG-3'
12. PDCD10 (851-176)
5'-ATAGATCTGTCCAGACTGCGCCTCT-3'
5'-CGAAGCTTTGGGTGGTGACACTTCT-3'



Heterologous promoter constructs
1. SERPINI1 (176-851)/S
5'-CCTGCTAGCTGGGTGGTGACACTTCTAG-3'
5'-AAAGATCTGTCCAGACTGCGCCTCTG-3'
2. PDCD10 (851-176)/S
5'-TAAGCTAGCGTCCAGACTGCGCCTCTG-3'
5'-CCTAGATCTTGGGTGGTGACACTTCTAG-3'
3. PDCD10 (473-176)/S
5'-TATGCTAGCCAAGCCCTCCCTTCACCTGG-3'
5'-CCTAGATCTTGGGTGGTGACACTTCTAG-3'
4. PDCD10 (710-474)/S
5'-GCTGCTAGCGTACCTTTCCGCTCCCTG-3'
5'-GAAGATCTGTGTGGGGACTTGCACTG-3'
5. PDCD10 (851-711)/S
5'-TAAGCTAGCGTCCAGACTGCGCCTCTG-3'
5'-TTAGATCTCAGGGCAGCTGCTTTCCG-3'
6. SERPINI1 (176-473)/C
5'-CCTGCTAGCTGGGTGGTGACACTTCTAG-3'
5'-CCAGATCTCAAGCCCTCCCTTCACCTGG-3'
7. SERPINI1 (474-710)/C
5'-GGAGCTAGCGTGTGGGGACTTGCA-3'
5'-CTAGATCTGTACCTTTCCGCTCCCTG-3'
8. SERPINI1 (711-851)/C
5'-CTTGCTAGCCAGGGCAGCTGCTTTCCG-3'
5'-AAAGATCTGTCCAGACTGCGCCTCTG-3'
*Construct names with an "S" character at the end indicates that the PCR-amplified intergenic product was cloned into the upstream of SV40 minimal promoter. Construct names with a "C" character denotes that the amplified intergenic segment was cloned into the upstream of CMV promoter.

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