Denk W, Strickler JH, Webb WW. Two-photon laser scanning fluorescence microscopy. Science. 1990; 248: 73–76.
Rubart M, Pasumarthi KB, Nakajima H, Soonpaa MH, Nakajima HO, Field LJ. Physiological coupling of donor and host cardiomyocytes after cellular transplantation. Circ Res. 2003; 92: 1217–1224.
Rubart M, Soonpaa MH, Nakajima H, Field LJ. Spontaneous and evoked intracellular calcium transients in donor-derived myocytes following intracardiac myoblast transplantation. J Clin Invest. 2004; 114: 775–783.
Helmchen F, Svoboda K, Denk W, Tank DW. In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons. Nat Neurosci. 1999; 2: 989–996.
Helmchen F, Waters J. Ca2+ imaging in the mammalian brain in vivo. Eur J Pharmacol. 2002; 447: 119–129.
Lichtman JW, Fraser SE. The neuronal naturalist: watching neurons in their native habitat. Nat Neurosci. 2001; 4 (suppl): 1215–1220.
Brown EB, Shear JB, Adams SR, Tsien RY, Webb WW. Photolysis of caged calcium in femtoliter volumes using two-photon excitation. Biophys J. 1999; 76: 489–499.
DelPrincipe F, Egger M, Niggli E. Calcium signalling in cardiac muscle: refractoriness revealed by coherent activation. Nat Cell Biol. 1999; 1: 323–329.
Soeller C, Cannell MB. Estimation of the sarcoplasmic reticulum Ca2+ release flux underlying Ca2+ sparks. Biophys J. 2002; 82: 2396–2414.
Svoboda K, Tank DW, Denk W. Direct measurement of coupling between dendritic spines and shafts. Science. 1996; 272: 716–719.
Brown EB, Wu ES, Zipfel W, Webb WW. Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery. Biophys J. 1999; 77: 2837–2849.
Zipfel WR, Williams RM, Webb WW. Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol. 2003; 21: 1369–1377.
Gauderon R, Lukins PB, Sheppard CJR. Effect of a confocal pinhole in two-photon microscopy. Microsc Res Tech. 1999; 47: 210–214.
Koester HJ, Baur D, Uhl R, Hell SW. Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage. Biophys J. 1999; 77: 2226–2236.
Hopt A, Neher E. Highly nonlinear photodamage in two-photon fluorescence microscopy. Biophys J. 2001; 80: 2029–3206.
Xu C, Webb WW. Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm. J Opt Soc Am B. 1996; 13: 481–491.
Patterson GH, Piston DW. Photobleaching in two-photon excitation microscopy. Biophys J. 2000; 78: 2159–2162.
Oliver AE, Baker GA, Fugate RD, Tablin F, Crowe JH. Effects of temperature on calcium-sensitive fluorescent probes. Biophys J. 2000; 78: 2116–2126.
Theer P, Hasan MT, Denk W. Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier. Opt Lett. 2003; 28: 1022–1024.
Centonze VE, White JG. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J. 1998; 75: 2015–2024.
Soeller C, Cannell MB. Two-photon microscopy: imaging in scattering samples and three-dimensionally resolved flash photolysis. Microsc Res Tech. 1999; 47: 182–195.
Periasamy A, Skoglund P, Noakes C, Keller R. An evaluation of two-photon versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis. Microsc Res Tech. 1999; 47: 172–181.
Oheim M, Beaurepaire, Chaigneau E, Mertz J, Charpak S. Two-photon microscopy in brain tissue: parameters influencing the imaging depth. J Neurosci Methods. 2001; 111: 29–37.
Levene MJ, Dombeck DA, Kasischke KA, Molloy RP, Webb WW. In vivo multiphoton microscopy of deep brain tissue. J Neurophysiol. 2004; 91: 1908–1912.
Rubart M, Wang E, Dunn KW, Field LJ. Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse hearts. Am J Physiol Cell Physiol. 2003; 284: C1654–C1658.
Kleinfeld D, Mitra PP, Helmchen F, Denk W. Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. Proc Natl Acad of Sci U S A. 1998; 95: 15741–15746.
Niggli E, Piston DW, Kirby MS, Cheng H, Sandison DR, Webb WW, Lederer WJ. A confocal laser scanning microscope designed for indicators with ultraviolet excitation wavelengths. Am J Physiol Cell Physiol. 1994; 266: C303–C310.
Kasischke KA, Vishwasrao HD, Fisher PJ, Zipfel WR, Webb WW. Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis. Science. 2004; 305: 99–103.
Despa S, Kockskamper J, Blatter LA, Bers DM. Na/K Pump-Induced [Na]i gradients in rat ventricular myocytes measured with two-photon microscopy. Biophys J. 2004; 87: 1360–1368.
Denk W. Two-photon scanning photochemical microscopy: mapping ligand-gated ion channel distributions. Proc Natl Acad of Sci U S A. 1994; 91: 6629–6633.
Matsuzaki M, Ellis-Davies GC, Nemoto T, Miyashita Y, Iino M, Kasai H. Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons. Nat Neurosci. 2001; 4: 1086–1092.
Fan GY, Fujisaki H, Miyawaki A, Tsay RK, Tsien RY, Ellisman MH. Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons. Biophys J. 1999; 76: 2412–2420.
Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science. 2003; 300: 1434–1436.
Wokosin DL, Loughrey CM, Smith GL. Characterization of fura dyes with two-photon excitation. Biophys J. 2004; 86: 1726–1738.
Xu C, Zipfel W, Shear JB, Williams RM, Webb WW. Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc Natl Acad of Sci U S A. 1996; 93: 10763–10768.
Hanson GT, McAnaney TB, Park ES, Rendell ME, Yarbrough DK, Chu S, Xi L, Boxer SG, Montrose MH, Remington SJ. Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application. Biochemistry. 2002; 41: 15477–15488.
Potter SM, Wang CM, Garrity PA, Fraser SE. Intravital imaging of green fluorescent protein using two-photon laser-scanning microscopy. Gene. 1996; 173: 25–31.
Sako Y, Sekihata A, Yanagisawa Y, Yamamoto M, Shimada Y, Ozaki K, Kusumi A. Comparison of two-photon excitation laser scanning microscopy with UV-confocal laser scanning microscopy in three-dimensional calcium imaging using the fluorescence indicator Indo-1. J Microsc. 1997; 185: 9–20.
Kuhn B, Fromherz P, Denk W. High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the red spectral edge. Biophys J. 2004; 87: 631–639.
Wang X, Krebs LJ, Al-Nuri M, Pudavar HE, Ghosal S, Liebow C, Nagy AA, Schally AV, Prasad PN. A chemically labeled cytotoxic agent: two-photon fluorophore for optical tracking of cellular pathway in chemotherapy. Proc Natl Acad of Sci U S A. 1999; 96: 11081–11084.
Hadjantonakis AK, Dickinson ME, Fraser SE, Papaioannou VE. Technicolour transgenics: imaging tools for functional genomics in the mouse. Nat Rev Genet. 2003; 4: 613–625.
Lansford R, Bearman G, Fraser SE. Resolution of multiple green-fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy. J Biomed Opt. 2001; 6: 311–318.
Neher E, Neher R. Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. Biophys J. 2004; 86: 318a.
Hadley RW, Lederer WJ. Ca2+ and voltage inactivate Ca2+ channels in guinea-pig ventricular myocytes through independent mechanisms. J Physiol. 1992; 444: 257–268.
Lipp P, Luscher C, Niggli E. Photolysis of caged compounds characterized by ratiometric confocal microscopy: a new approach to homogeneously control and measure the calcium concentration in cardiac myocytes. Cell Calcium. 1996; 19: 255–266.
Cheng H, Lederer WJ, Cannell MB. Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle. Science. 1993; 262: 740–744.
McCray JA, Trentham DR. Properties and uses of photoreactive caged compounds. Annu Rev Biophys Biophys Chem. 1989; 18: 239–270.
Jacobs MD, Soeller C, Sisley AM, Cannell MB, Donaldson PJ. Gap junction processing and redistribution revealed by quantitative optical measurements of connexin46 epitopes in the lens. Invest Ophthalmol Vis Sci. 2004; 45: 191–199.
Mulligan SJ, MacVicar BA. Calcium transients in astrocyte endfeet cause cerebrovascular constrictions. Nature. 2004; 431: 195–199.
Stroh M, Zipfel WR, Williams RM, Webb WW, Saltzman WM. Diffusion of nerve growth factor in rat striatum as determined by multiphoton microscopy. Biophys J. 2003; 85: 581–588.
Gaudesius G, Miragoli M, Thomas SP, Rohr S. Coupling of cardiac electrical activity over extended distances by fibroblasts of cardiac origin. Circ Res. 2003; 93: 421–428.
Peters NS, Coromilas J, Severs NJ, Wit AL. Disturbed connexin43 gap junctional distribution correlates with the location of reentrant circuits in the epicardial border zone of healing canine infarcts that cause ventricular tachycardia. Circulation. 1997; 95: 988–996.
Akar FG, Spragg DD, Tunin RS, Kass DA, Tomaselli GF. Mechanisms underlying conduction slowing and arrhythmogenesis in nonischemic dilated cardiomyopathy. Circ Res. 2004; 95: 717–725.
Vega RB, Bassel-Duby R, Olson EN. Control of cardiac growth and function by calcineurin signaling. J Biol Chem. 2003; 278: 36981–36984.
Chaigneau E, Oheim M, Audinat E, Charpak S. Two-photon imaging of capillary blood flow in olfactory bulb glomeruli. Proc Natl Acad of Sci U S A. 2003; 100: 13081–13086.
Dunn KW, Sandoval RM, Kelly KJ, Dagher PC, Tanner GA, Atkinson SJ, Bacallao RL, Molitoris BA. Functional studies of the kidney of living animals using multicolor two-photon microscopy. Am J Physiol Cell Physiol. 2002; 283: C905–C916.
Miller MJ, Wei, SH, Parker I, Cahalan MD. Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science. 2002; 296: 1869–1873.
Brown EB, Campbell RB, Tsuzuki Y, Xu L, Carmeliet P, Fukumura D, Jain RK. In vivo measurement of gene expression, angiogenesis and physiological function in tumors using multiphoton laser scanning microscopy. Nat Med. 2001; 7: 864–868.
Wier WG, ter Keurs HE, Marban E, Gao WD, Balke CW. Ca2+ ‘sparks’ and waves in intact ventricular muscle resolved by confocal imaging. Circ Res. 1997; 81: 462–469.
Satoh H, Delbridge LMD, Blatter L, Bers DM. Surface:volume relationship in cardiac myocytes studied with confocal microscopy and membrane capacitance measurements: species-dependence and developmental effects. Biophys J. 1996; 98: 1236–1248.
Ito K, Yan X, Tajima M, Su Z Barry WH, Lorell BH. Contractile reserve and intracellular calcium regulation in mouse ventricular myocytes from normal and hypertrophied failing hearts. Circ Res. 2000; 87: 588–595.
Heinzel FR, Bito V, Volders PG, Antoons G, Mubagwa K, Sipido KR. Spatial and temporal inhomogeneities during Ca2+ release from the sarcoplasmic reticulum in pig ventricular myocytes. Circ Res. 2002; 91: 1023–1030.
Cordeiro JM, Greene L, Heilmann C, Antzelevitch D, Antzelevitch C. Transmural heterogeneity of calcium activity and mechanical function in the canine left ventricle. Am J Physiol Heart Circ Physiol. 2004; 286: H1471–H1479.
Cordeiro JM, Spitzer KW, Giles WR, Ershler PE, Cannell MB, Bridge JH. Location of the initiation site of calcium transients and sparks in rabbit heart Purkinje cells. J Physiol. 2001; 531: 301–314.
Boyden PA, Pu J, Pinto J, Keurs HE. Ca(2+) transients and Ca(2+) waves in Purkinje cells: role in action potential initiation. Circ Res. 2000; 86: 448–455.
Litwin SE, Zhang D, Bridge JHB. Dyssynchronous Ca2+ Sparks in Myocytes From Infarcted Hearts. Circ Res. 2000; 87: 1040–1047.
Rocheleau JV, Head WS, Nicholson WE, Powers AC, Piston DW. Pancreatic islet beta-cells transiently metabolize pyruvate. J Biol Chem. 2002; 277: 30914–30920.
Patterson GH, Knobel SM, Arkhammar P, Thastrup O, Piston DW. Separation of the glucose-stimulated cytoplasmic and mitochondrial NAD(P)H responses in pancreatic islet beta cells. Proc Natl Acad of Sci U S A. 2000; 97: 5203–5207.
Brandes R, Bers DM. Simultaneous measurements of mitochondrial NADH and Ca2+ during increased work in intact rat heart trabeculae. Biophys J. 2002; 83: 587–604.
Aon MA, Cortassa S, Marban E, O’Rourke B. Synchronized whole cell oscillations in mitochondrial metabolism triggered by a local release of reactive oxygen species in cardiac myocytes. J Biol Chem. 2003; 278: 44735–44744.
Squirrell JM, Wokosin DL, White JG, Bavister BD. Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability. Nat Biotechnol. 1999; 17: 763–767.
Tirlapur UK, Konig K. Targeted transfection by femtosecond laser. Nature. 2002; 418: 290–291.
Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science. 2004; 305: 1007–1009.
Schwille P, Haupts U, Maiti S, Webb WW. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys J. 1999; 77: 2251–2265.
Becker W, Bergmann A, Hink MA, Koenig K, Benndorf K, Biskup C. Fluorescence lifetime imaging by time-correlated single-photon counting. Microsc Res Tech. 2004; 63: 58–66.