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Figs 6–14. Trichoderma cultures grown on PDA in 9-cm-diam Petri dishes for 96 h at 25 °C under 12 h darkness/12 h cool white fluorescent light. 6: T. austrokoningii G.J.S. 99-146. 7. T. austrokoningii (New Zealand) G.J.S. 99-116. 8. T. austrokoningii (Russia) G.J.S. 00-73. 9. T. caribbaeum var. caribbaeum G.J.S. 97-3. 10. T. dingleyae G.J.S. 99-105. 11. T. dorotheae G.J.S. 99-97. 12–13. T. intricatum (12: G.J.S. 96-13, 13: G.J.S. 97-88). 14. T. koningii G.J.S. 96-119.
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Figs 15–23. Trichoderma cultures grown on PDA in 9-cm-diam Petri dishes for 96 h at 25 °C under 12 h darkness/12 h cool white fluorescent light. 15–16. T. koningiopsis (15: G.J.S. 01-07, 16: G.J.S. 91-6). 17. T. ovalisporum DIS 172h. 18. T. petersenii DAOM 165782. 19–20. T. rogersonii (19: G.J.S. 90-125; 20: G.J.S. 92-116). 21–22. T. stilbohypoxyli (21: G.J.S. 02-143, in darkness; 22: G.J.S. 03-103, 7 d). 23. T. taiwanense G.J.S. 95-93.
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Figs 24–35. Hypocrea teleomorphs of Trichoderma species. 24–27. T. austrokoningii. 24–25 from type, 26 from Florida (C.T.R. 85-57), 27 from Russia (G.J.S. 00-73). 28–29. T. caribbaeum var. caribbaeum on stroma of?Penzigia sp. (G.J.S. 97-3). 30–31. T. dingleyae immature (30: G.J.S. 02-50) and mature (31: G.J.S. 99-105) stromata. 32–33. T. dorotheae mature and immature (arrow) stromata (32: G.J.S. 99-97, 33: G.J.S. 99-194). 34–35. T. intricatum immature (34: G.J.S. 96-13, Puerto Rico) and mature (35: G.J.S. 97-88, Thailand) stromata. Microscopy: all stereo. Bars: 24–25, 27–28, 30–34 = 1 mm; 26, 29, 35 = 0.5 mm.
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Figs 36–50. Hypocrea teleomorphs of Trichoderma species. 36–38. T. koningii (36, 38: G.J.S. 89-122, 37: G.J.S. 00-156). 39–40. T. koningiopsis (G.J.S. 93-20, type). 41–43. T. petersenii (41–42: G.J.S. 98-139; 43 G.J.S. 04-355 France), immature stromata shown in 41. 44–46. T. rogersonii. Immature stroma (arrow) in 44 (44: G.J.S. 95-217; 45: G.J.S. 90-79; 46: G.J.S. 90-125). 47–48. T. stilbohypoxyli (47: G.J.S. 96-43, 48: G.J.S. 03-103, immature). 49–50. T. taiwanense, overmature stromata (G.J.S. 95-93, type). Microscopy: all stereo. Bars: 36 = 2 mm; 37–39, 41, 44, 46, 47–49 = 1 mm; 40, 42–43, 45, 50 = 0.5 mm.
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Figs 51–59. T. austrokoningii, anamorph (Queensland, including type; all from CMD). 51–52. Conidial pustules. 53–58. Conidiophores and phialides. Intercalary phialide shown in 58 (arrow). 59. Conidia. Figs 51, 53–55, 59 from G.J.S. 99-147; 52, 56–58 from G.J.S. 99-146. Microscopy: 51–52 = stereo; 53–56 = PC; 57–59 = DIC. Bars: 51 = 1 mm; 52 = 0.5 mm; 53–58 = 20 µm; 59 = 10 µm.
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Figs 60–70. T. austrokoningii, Hypocrea teleomorph (Queensland, including type). 60. Median longitudinal section of a mature perithecium. 61. Surface view of stroma showing two ostiolar openings (arrow) and small cells that form hairs. 62–63. Hairs arising from stroma surface (arrows). 64. Section of stroma surface showing hairs (arrows) and pigmented outer region. 65. Median longitudinal section through an ostiolar canal. 66. Cells of stroma interior below perithecia. 67–68. Asci. Apical ring visible in 68–70. Discharged ascospores. Spores shown in optical section in 69, surface view showing ornamentation in 70. Figs 60, 63–64, 66–67, 69–70 from G.J.S. 99-147; 61–62, 65, 68 from G.J.S. 99-146. Microscopy: 60 = BF; all others DIC. Bars: 60 = 100 µm; 61–68 = 20 µm; 69–70 = 10 µm.
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Figs 71–79. (Page 100). Trichoderma austrokoningii from Russia (G.J.S. 00-73) on CMD. 71–72. Conidial pustules. Note long, entirely fertile conidiophores in 72 (examples marked with arrows). 73–78. Conidiophores and phialides. Intercalary phialides indicated by arrows in 78. 79. Conidia. Microscopy: 71–72 = stereo; 73–76, 78 = PC; 77, 79 = DIC. Bars: 71 = 1 mm; 72 = 0.5 mm; 73–78 = 20 µm; 79 = 10 µm.
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Figs 80–88. Trichoderma austrokoningii from New Zealand on CMD. 80. Aggregated conidiophores. 81–87. Conidiophores. 88. Conidia. Figs 80–84, 86–87 from G.J.S. 99-116; 85, 88 from CBS 243.63. Microscopy: 80–81 = stereo; 82–85 = PC; 86–88 = DIC. Bars: 80 = 1 mm; 81 = 0.5 mm; 82–87 = 20 µm; 88 = 10 µm.
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Figs 89–101. Trichoderma austrokoningii from Florida and Taiwan on CMD. 89–91. Conidial pustules. 92–99. Conidiophores. 100–101. Conidia. Figs 89, 91, 97–99, 101 from G.J.S. 96-163; 90, 92-96, 100 from C.T.R. 85-57. Microscopy: 89–91 = stereo; 92–99 = PC; 100–101 = DIC. Bars: 89–90 = 1 mm; 91 = 0.5 mm; 92–99 = 20 µm; 100–101 = 10 µm.
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Fig. 102. Growth curves of variants of T. austrokoningii. Solid line = PDA, broken line = SNA. The following isolates were included: Australia (G.J.S. 99-146, G.J.S. 99-174), New Zealand (G.J.S. 99-116, CBS 243.63), Russia (G.J.S. 00-73), U.S.A./Taiwan (C.T.R. 85-57, G.J.S. 96-163). Standard error bars are shown.
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