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The authors studied tissue engineering in space by using cartilage as a …


Biology Articles » Bioengineering » Tissue engineering of cartilage in space » Table

Table
- Tissue engineering of cartilage in space

 


Parameter Tissue engineered constructs
Natural cartilage**
At launch* Mir-grown Earth-grown

Culture time (months) 3 7 7 0
Chondrocyte function
  Sulfate incorporation (ng/µg DNA/day) 80.8 ± 37.2 (3) 88.9 ± 8.0 (3) 83.4 ± 13.4 (3) 332 ± 36.3 (7)
  Proline incorporation (ng/µg DNA/day) 82.0 ± 20.6 (3) 93.0 ± 3.0 (3) 87.9 ± 22.7 (3) 205 ± 46.8 (7)
Construct composition  
  Weight (mg wet) 250 ± 38 (20) 330 ± 25 (5) 429 ± 14 (5) 461 ± 72.8 (7)
  Water (%) 89.2 ± 0.89 (3) 89.2 ± 0.31 (3) 86.3 ± 0.53 (3) 82.8 ± 0.93 (7)
  Cells (% wet weight) 0.64 ± 0.03 (3) 0.40 ± 0.01 (3) 0.46 ± 0.02 (3) 0.66 ± 0.09 (7)
  GAG (% wet weight) 6.03 ± 0.84 (3) 3.59 ± 0.22 (3) 8.83 ± 0.93 (3) 7.05 ± 0.56 (6)
  Collagen (% wet weight) 2.70 ± 0.75 (3) 3.42 ± 0.17 (3) 3.68 ± 0.27 (3) 10.7 ± 0.91 (6)
  Total of above components (% wet weight) 98.6 ± 0.07 (3) 96.6 ± 0.68 (3) 99.3 ± 0.60 (3) 101 ± 0.46 (6)
  Type II collagen (% total collagen) 91.6 ± 19.1 (2) 78.0 ± 4.1 (4) 75.3 ± 7.8 (4) 90.3 ± 17.9 (5)
Mechanical behavior
  Aggregate modulus (MPa) 0.108 ± 0.047 (2) 0.313 ± 0.045 (4) 0.932 ± 0.049 (3) 0.949 ± 0.021 (3)
  Hydraulic permeability (×10-15 m4/Ns) 8.25 ± 1.94 (2) 6.73 ± 3.02 (4) 3.72 ± 0.167 (3) 2.72 ± 0.641 (3)
  Dynamic stiffness (MPa, 1 Hz) 2.23 ± 0.12 (2) 3.80 ± 0.39 (4) 7.75 ± 0.30 (3) 16.8 ± 1.14 (3)

Data represent average ± standard deviation. Parentheses indicate the number of samples analyzed per group.
Statistical significance was assessed using ANOVA (alpha = 0.05) in conjunction with Tukey's studentized range test. For each parameter, statistically significant differences between groups (P 
* Wet weights at 3 months were those of the actual constructs used in 7-month Mir and Earth cultivations; all other parameters in this group were assessed using additional 3-month constructs from the same batch.
** Wet weights for natural cartilage were scaled by a factor of five for better comparison with corresponding values measured for Earth-grown tissue engineered constructs.


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