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The authors studied tissue engineering in space by using cartilage as a …


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Figures
- Tissue engineering of cartilage in space

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Figure 1. Experimental design. Cartilage cells were seeded onto polymer scaffolds, and the resulting constructs were cultivated in rotating bioreactors first for 3 months on Earth and then for 4 additional months either on the Mir Space Station (10-4-10-6 g) or on Earth (1 g). 

Figure 1

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Figure 2. Cell viability postflight. Cells isolated from a 7-month construct from the Mir group after 2 days of monolayer culture. (×200.) Intracellular esterase activity is indicated by green color. 

Figure 2

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Figure 3. Construct structure. (A and B) Full cross sections of constructs from Mir and Earth groups. (×10.) (C and D) Representative areas at the construct surfaces. (×200.) GAG is stained red with safranin-O. 

Figure 3

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Figure 4. Construct ultrastructure. Transmission electron micrographs of constructs from Mir (A) and Earth (B) groups at magnifications of ×3,500 and ×120,000 (Inset).

Figure 4

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