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This is the first demonstration of an enzyme activity transferring a nucleotidyl …


Biology Articles » Biochemistry » Enzymology » Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate » Table

Table
- Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate

Partial purification of AThTP-synthesizing enzyme from E. coli

Fraction
Volume (ml)
Total protein (mg)
Total activity (pmol.min-1)
Specific activity (pmol.min-1.mg-1)
Purification factor
Extract
32.0
99.2
436.5
4.4
1
Heat treatment
31.0
71.3
370.8
5.2
1.2
Sephadex G-200:





Peak I (maximum)
4.0
0.68
63.4
93.2
21.2
Peak II (maximum)
4.0
0.94
75.4
80.2
18.2

The purification factor was calculated by comparing the activity in a given fraction to the activity found in the extract.


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