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Biology Articles » Methods & Techniques » TCA Precipitation

TCA Precipitation

TCA Precipitiation of proteins

Used for concentration of proteins for subsequent gel electrophoresis.

  • Add TCA (Trichloro acetic acid) to a final concentration of 10%
  • Incubate on ice for 30 minutes
  • Spin down precipitated material at 4 degrees at 20 000 x g for 30 minutes
  • Remove supernatant
  • Add 300 ul ice-cold acetone to wash and spin as above for 5 minutes
  • Remove supernatant
  • Air dry the pellet
  • Resuspend in SDS-PAGE loading buffer or other preffered buffer.
Source: YourScicom.com



rating: 3.00 from 4 votes | updated on: 21 Aug 2007 | views: 7319 |

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