In an effort to investigate how loss of deneddylation affects SCF activity, …

• Abstract
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• Methods
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Biology Articles » Biochemistry » Protein Biochemistry » Targeted silencing of Jab1/Csn5 in human cells downregulates SCF activity through reduction of F-box protein levels » Abbreviations

Abbreviations

- Targeted silencing of Jab1/Csn5 in human cells downregulates SCF activity through reduction of F-box protein levels

Abbreviations

CSN: COP9/signalosome

SCF: SKP1/Cullin/F-box protein complex

JAMM: Jab1/MPN domain metalloenzyme motif

BTB: "bric-a-brac/tramtrack/broad" protein complex

Authors' contributions

All experiments were performed by GC. Both GC and RJD conceived and designed the experiments. Both authors contributed to the writing of the manuscript and both authors read and approved the final manuscript.

Acknowledgements

We thank W. Krek, W. Boelens, B. Clurman and S. Reed for generously providing antibodies and advice. We thank Nagesh Rao for performing FISH and kareotype analysis. We thank Rati Verma, Matt Petroski, Thibault Mayor and other members of the Deshaies lab for providing helpful insight and discussions during the course of this work.