Table
- Sugar recognition by human galactokinase

Table 1. Kinetic parameters of the mutant galactokinases. All reactions were carried out at 37°C as described in the materials and methods. Values were derived by non-linear curve fitting as implemented in the program GraphPad Prism and are shown ± standard error as derived from this procedure.

Mutant	k_cat/s^-1	K_m,gal/μM	K_m,ATP/μM	k_cat/K_m,gal/l.mol^-1.s^-1	k_cat/K_m,ATP/10⁵× l.mol^-1.s^-1

Wild Type⁽¹⁾	8.7 ± 0.5	970 ± 220	34 ± 4	8900 ± 2900	2.6 ± 0.4
E43A	6.7 ± 0.02	1900 ± 500	35 ± 0.3	3400 ± 430	1.9 ± 0.009
E43G	0.9 ± 0.02	140 ± 10	3.9 ± 0.6	6100 ± 330	2.4 ± 0.3
H44A				Not soluble
H44I				Not soluble
E43G/H44I				Not soluble
D46A				No detectable activity

Notes ⁽¹⁾Previously reported in [12]

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Source: BMC Biochemistry 2003, 4:16

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Table - Sugar recognition by human galactokinase

Table
- Sugar recognition by human galactokinase