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Since only studies on human enzyme are likely to be of value …


Biology Articles » Biochemistry » Sugar recognition by human galactokinase » Table

Table
- Sugar recognition by human galactokinase

Table 1. Kinetic parameters of the mutant galactokinases. All reactions were carried out at 37°C as described in the materials and methods. Values were derived by non-linear curve fitting as implemented in the program GraphPad Prism and are shown ± standard error as derived from this procedure.
Mutant
kcat/s-1
Km,gal/μM
Km,ATP/μM
kcat/Km,gal/l.mol-1.s-1
kcat/Km,ATP/105 × l.mol-1.s-1
Wild Type(1)
8.7 ± 0.5
970 ± 220
34 ± 4
8900 ± 2900
2.6 ± 0.4
E43A
6.7 ± 0.02
1900 ± 500
35 ± 0.3
3400 ± 430
1.9 ± 0.009
E43G
0.9 ± 0.02
140 ± 10
3.9 ± 0.6
6100 ± 330
2.4 ± 0.3
H44A



Not soluble

H44I



Not soluble

E43G/H44I



Not soluble

D46A



No detectable activity

Notes (1) Previously reported in [12]

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Source: BMC Biochemistry 2003, 4:16 


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