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S. griseus carries a single close homologue of atrA and that expression …
Biology Articles » Biotechnology » Red Biotechnology » Streptomycin production by Streptomyces griseus can be modulated by a mechanism not associated with change in the adpA component of the A-factor cascade
|(1)||Faculty of Biological Sciences, University of Leeds, Leeds, LS8 2BH, UK|
|(2)||Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing, 100050, China|
|(3)||Institute for Innovation and Development of Learning Process, Mahdiol University, Rama VI, Bangkok, 10400, Thailand|
|(4)||Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G1 1XW, UK|
In Streptomyces coelicolor, AtrA is an activator of transcription of the actinorhodin cluster-situated regulator gene actII-ORF4. In previous work, we showed that S. coelicolor AtrA binds in vitro to the promoter of S. griseus strR, the streptomycin cluster-situated regulator. We show here that S. griseus carries a single close homologue of atrA and that expression of S. coelicolor AtrA in S. griseus causes a DNA binding-dependent reduction in streptomycin production and in the mRNA levels of strR and genes of streptomycin biosynthesis. However, there is no effect on the level of the mRNA of adpA, which is the only transcription factor that has so far been characterised for strR. The adpA gene is directly regulated by ArpA, the receptor protein for the γ-butyrolactone signalling molecule A-factor. Therefore, to our knowledge, our results provide the first in vivo evidence that A-factor-ArpA-AdpA-StrR regulatory cascade represents only part of the full complexity of regulation of streptomycin biosynthesis in S. griseus. The potential biotechnological application of our findings is discussed.
Keywords Actinorhodin - Secondary metabolite production - Streptomyces gene regulation - S. coelicolor - S. griseus - Streptomycin
Biotechnology Letters. © Springer Science+Business Media B.V. 2006.10.1007/s10529-006-9216-2.
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