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Spermatozoa or spermatids, retrieved from frozen reproductive organs or frozen bodies of …
Biology Articles » Cryobiology » Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring » Figures
Fig. 1. Freezing epididymides and testes. (A) Epididymides (small arrow) and testes (large arrow) removed from adult male mice; two cryotubes (upper left) and a freezing container (Bicell, upper right). (B) Epididymides and testes are put in cryotubes before being placed in a freezing container. (C) Freezing containers stored in a freezer.
Fig. 2. Spermatozoa and spermatogenic cells collected from a testis frozen at –80°C for 1 month. Haploid germ cells can be identified by their size and nuclear shape, although their plasma membrane is disintegrated extensively. (Insets, from the left) A presumptive round spermatid, an early elongated spermatid, and an elongated spermatid. (Bar, 10 µm.)
Fig. 3. Temperature changes inside cryotubes in different freezing protocols. Temperature declines most slowly when the cryotube is placed in a freezing container and is fastest when a cryotube is plunged directly into liquid nitrogen (LN2).
Fig. 4. Production of mice using spermatozoa retrieved from testes of male mice frozen for 15 years. (A) Spermatozoa collected from seminiferous tubules show severe structural damages. The head (arrows) and midpiece (arrowheads) have cellular debris. (B) Pups born after injection of frozen spermatozoa (BALB/c-nude) into B6D2F1 oocytes. Because BALB/c and B6D2F1 have the coat-color genotype of A/A b/b c/c D/D and A/a B/b C/C D/D, respectively, the offspring are agouti (heterozygous for all loci; #1) or brown (homologous for the b locus; #2). The albino mouse is their foster mother.
Source: PNAS, August 29, 2006, vol. 103, no. 35, 13098-13103.
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