Serum butyrylcholinesterase in type 2 diabetes mellitus: a biochemical and bioinformatics approach
GR Sridhar2, G Nirmala2, Allam Apparao1, AS Madhavi1, S Sreelatha1, J Sudha Rani1 and P Vijayalakshmi1
1Department of Computer Science and Systems Engineering, Andhra University, Visakhapatnam, India
2Endocrine and Diabetes Centre, 15-12-16 Krishnanagar, Visakhapatnam 530 002, India
Open access article from Lipids in Health and Disease 2005,
Butyrylcholinesterase is an enzyme that may serve as a marker of
metabolic syndrome. We (a) measured its level in persons with diabetes
mellitus, (b) constructed a family tree of the enzyme using nucleotide
sequences downloaded from NCBI. Butyrylcholinesterase was estimated
colorimetrically using a commercially available kit (Randox Lab, UK). Phylogenetic trees were constructed by distance method (Fitch and Margoliash method) and by maximum parsimony method.
There was a negative correlation between serum total cholesterol and
butyrylcholinesterase (-0.407; p < 0.05) and between serum LDL
cholesterol and butyrylcholinesterase (-0.435; p < 0.05). There was
no statistically significant correlation among the other biochemical
parameters. In the evolutionary tree construction both methods gave
similar trees, except for an inversion in the position of Sus scrofa (M62778) and Oryctolagus cuniculus (M62779) between Fitch and Margoliash, and maximum parsimony methods.
The level of butyrylcholinesterase enzyme was inversely related to
serum cholesterol; dendrogram showed that the structures from
evolutionarily close species were placed near each other.
Keywords: metabolic syndrome, type 2 diabetes, dyslipidemia, phylogenetic tree, evolutionary distance