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Rhizopus oryzae glucoamylase (RoGA) consists of three domains: an amino (N)-terminal …
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- Role of the linker region in the expression of Rhizopus oryzae glucoamylase
An GA, Aspergillus niger glucoamylase; Ro GA, Rhizopus oryzae glucoamylase; CBM, carbohydrate-binding module; SBD, starch-binding domain
SCL designed and generated most of the mutants, carried out most functional assays, and drafted the manuscript; WTL expressed and purified recombinant SBDs. SHL involved initial cloning and enzymatic assay designs; WIC participated in the structural determination employing spectroscopic methodologies; BKH assisted recombinant yeast generation and growth. IPL participated in manuscript preparation. CCS participated in experimental design and coordination. MDTC conceived and supervised this project and manuscript writing. All authors read and approved the final manuscript.
This investigation was supported by grants from Simpson Biotech Co., Ltd., the Council of Agriculture and the National Science and Technology Program for Agricultural Biotechnology (96AS-1.2.1.-ST-a5).
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