The author(s) declare that they have no competing interests.
JRP conceived and contributed to the development of the NiV VLP system, designed and constructed all expression constructs and carried out all recombinant expression and analysis assays, interpreted data, and wrote the first draft of the manuscript. GC developed and carried out all live virus infections and density analysis and interpreted data and edited and corrected the manuscript. LFW edited and corrected the manuscript. BTE provided expertise for conducting the live virus infection experiments, financial support, edited and corrected the manuscript. CCB conceived and contributed to the development of the NiV VLP system, provided overall supervision and financial support and prepared the final versions of the manuscript.
The views expressed in the manuscript are solely those of the authors, and they do not represent official views or opinions of the Department of Defense or The Uniformed Services University of the Health Science. J.R. Patch performed this work as partial fulfillment of the requirements of the Ph.D. program in Emerging Infectious Diseases of the Uniformed Services University of the Health Sciences. We thank Jody Berry and Hana Weingartl for monoclonal antibodies, Robert Lamb for SV5 reagents, Michael Delannoy (Johns Hopkins University School of Medicine Microscope Facility) for electron microscopy assistance, and Katharine Bossart for reagents and assistance. We also thank Michael Flora and the Biomedical Instrumentation Center staff, as well as Andrew Hickey, Yan-ru Feng and Lemin Wang (Uniformed Services University) for assistance. This work was supported by the Middle Atlantic Regional Center of Excellence (MARCE) for Biodefense and Emerging Infectious Disease Research, NIH grant AI057168 and NIH AI054715 grant to C.C.B.