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Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC).


Biology Articles » Biotechnology » Quantification of the CBD-FITC conjugates surface coating on cellulose fibres » Conclusion

Conclusion
- Quantification of the CBD-FITC conjugates surface coating on cellulose fibres

In this work, the surface concentration of CBD adsorbed on cellulose fibres was estimated. The coating values obtained were higher than expected, corresponding in theory to several layers (4 to 6) of CBDs adsorbed on the external surface. However, it has been demonstrated that the CBDs penetrate the fibres. An important amount of CBDs was detected inside the fibres by immunolabelling and confocal microscopy. It seems that a large fraction of the adsorbed CBDs actually penetrate the fibres. The surface coverage values are, undoubtedly, high enough to justify a change in the fibres' surface properties. CBDs may therefore be used as powerful tools to modulate the fibres' surface properties.

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