..................................................
Table 1 |
General characterization of the amounts of material taken in the experiments
|
|
Experiment II
|
Experiment III
|
Experiment IV
|
|
|
|
μg
|
cpm
|
μg
|
cpm
|
μg
|
cpm
|
|
DNA* amount (μg and cpm) per point
|
1.4
|
1.08 × 107
|
3.6
|
2.04 × 107
|
2.5
|
1.8 × 107
|
α-dATP*amount (cpm) per point
|
|
0.9 × 107
|
|
2.0 × 107
|
|
1.4 × 107
|
|
..................................................
Table 2 |
Absolute counts (AC, cpm) and percent of the α-dATP*-labeled material added to culture medium
|
α-dATP* 180 min
|
Experiment II Exponential phase
|
Experiment III Exponential phase
|
Experiment IV Cell monolayer
|
|
Cytoplasmic fraction (cpm)
|
94 775
|
128 271
|
348 924
|
% of the amount added to medium
|
1.1%
|
0.64%
|
2.49%
|
Interchromosomal fraction (cpm)
|
31 328
|
13 886
|
128 212
|
% of the amount added to medium
|
0.35%
|
0.07%
|
0.88%
|
Chromatin (cpm)
|
5 915
|
14 689
|
32 516
|
% of the amount added to medium
|
0.066%
|
0.07%
|
0.22%
|
|
..................................................
Table 3
|
Quantitative characteristics of the labeled material content in various cell compartments depending on the time of its incubation with cells
|
Incubation time
|
0 min
|
15 min
|
30 min
|
60 min
|
120 min
|
180 min
|
|
Experiment no.
|
II
|
III
|
IV
|
II
|
III
|
IV
|
II
|
III
|
IV
|
II
|
III
|
IV
|
II
|
III
|
IV
|
II
|
III
|
IV
|
|
Cytoplasmic fraction (absolute counts, cpm)
|
74 883
|
470 559
|
275 481
|
542 304
|
|
|
674 216
|
|
|
677 588
|
980 421
|
854 765
|
|
960 000
|
1 060 674
|
518 282
|
863 345
|
368 101
|
% of the amount added to medium
|
0.7%
|
2.3%
|
1.5%
|
5.0%
|
|
|
6.2%
|
|
|
6.3%
|
4.8%
|
4.7%
|
|
4.7%
|
5.8%
|
4.8%
|
4.2%
|
2.0%
|
Amount in μg according to %
|
0.0098
|
0.08
|
0.0375
|
0.07
|
|
|
0.087
|
|
|
0.088
|
0.17
|
0.118
|
|
0.17
|
0.145
|
0.67
|
0.15
|
0.05
|
Interchromosomal fraction (absolute counts, cpm)
|
11 460
|
3060
|
7330
|
83 662
|
|
|
95 821
|
|
|
97 147
|
159 378
|
322 008
|
|
299 963
|
445 426
|
177 814
|
282 826
|
203 421
|
% of the amount added to medium
|
0.1%
|
0.015%
|
0.04%
|
0.77%
|
|
|
0.89%
|
|
|
0.9%
|
0.78%
|
1.75%
|
|
1.47%
|
2.42%
|
1.65%
|
1.39%
|
1.1%
|
Amount in μg according to %
|
0.0014
|
0.0005
|
0.0025
|
0.01
|
|
|
0.013
|
|
|
0.0135
|
0.03
|
0.04
|
|
0.053
|
0.061
|
0.023
|
0.05
|
0.028
|
% of the genome
|
0.066%
|
0.016%
|
0.083%
|
0.33%
|
|
|
0.43%
|
|
|
0.45%
|
1.0%
|
1.33%
|
|
1.77%
|
2.03%
|
0.73%
|
1.67%
|
0.92%
|
Chromatin (absolute counts, cpm)
|
8089
|
3691
|
2248
|
45 812
|
|
|
47 684
|
|
|
49 394
|
296 771
|
123 390
|
|
242 836
|
157 124
|
37 620
|
244 937
|
118 285
|
% of the amount added to medium
|
0.075%
|
0.018%
|
0.012%
|
0.4%
|
|
|
0.44%
|
|
|
0.46%
|
1.45%
|
0.67%
|
|
1.19%
|
0.85%
|
0.35%
|
1.2%
|
0.64%
|
Amount in μg according to %
|
0.0011
|
0.00065
|
0.0003
|
0.0056
|
|
|
0.006
|
|
|
0.0064
|
0.05
|
0.0168
|
|
0.0428
|
0.021
|
0.0042
|
0.043
|
0.016
|
|
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