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table of contents  Snake venom is a complex mixture of proteins and peptides, and a … |
Biology Articles » Zoology » Herpetology » Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys » Tables
Tables
|
| (a) | |||||
|---|---|---|---|---|---|
| Approach | Shotgun-LC-MS/MS (%) | 1DE-LC-MS/MS (%) | GF-LC-MS/MS (%) | GF-2DE-MALDI–TOF-MS (%) | All approaches (%) |
| Venom proteins | |||||
| Cardiotoxins | 56 (44) | 70 (46) | 78 (60) | − | 56 (68) |
| Neurotoxins | 5 (4) | 4 (2) | 13 (10) | − | 8 (11) |
| Haemotoxins | 3 (2) | 2 (1) | − | 13 (2) | 2 (3) |
| Phospholipases A2 | 18 (14) | 7 (5) | 4 (3) | − | 11 (15) |
| Metalloproteinases | − | − | − | − | − |
| Other proteins | 18 (14) | 17 (11) | 5 (4) | 87 (14) | 22 (27) |
| (b) | |||||
| Approach | Shotgun-LC-MS/MS (%) | 1DE-LC-MS/MS (%) | GF-LC-MS/MS (%) | GF-2DE-MALDI–TOF-MS (%) | All approaches (%) |
| Venom proteins | |||||
| Cardiotoxins | 7 (2) | 7 (3) | 8 (4) | − | 8 (6) |
| Neurotoxins | − | − | 2 (1) | − | 1 (1) |
| Haemotoxins | 28 (8) | 23 (10) | 32 (15) | 27 (4) | 35 (26) |
| Phospholipases A2 | 14 (4) | 23 (10) | 15 (7) | 27 (4) | 18 (13) |
| Metalloproteinases | 40 (11) | 30 (13) | 30 (14) | 33 (5) | 27 (20) |
| Other proteins | 11 (3) | 16 (7) | 13 (6) | 13 (2) | 11 (8) |
Table 2 Comparison of the identified venomous proteins and their sequence sources in database of snake proteins
All sequence data from snake venoms, cobra and viper, are from NCBI and EBI. The information of species sources are provided by submitters.
| Total identified proteins | Sequence source from cobra | Sequence source from viper | Sequence source from other species | ||
|---|---|---|---|---|---|
| Naja | Shotgun-LC-MS/MS | 78 | 70 (90%) | 2 (2%) | 6 (8%) |
| 1DE-LC-MS/MS | 65 | 57 (87%) | 1 (1.5%) | 7 (10.7%) | |
| GF-LC-MS/MS | 77 | 76 (98.7%) | − | 1 (1.3%) | |
| GF-2DE-MALDI–TOF-MS | 16 | 5 (31%) | 1 (6.3%) | 10 (62.5%) | |
| Agki | Shotgun-LC-MS/MS | 28 | 1 (3.5%) | 26 (93%) | 1 (3.5%) |
| 1DE-LC-MS/MS | 43 | 1 (2%) | 42 (98%) | − | |
| GF-LC-MS/MS | 47 | 1 (1.5%) | 46 (97%) | 1 (1.5%) | |
| GF-2DE-MALDI–TOF-MS | 15 | − | 14 (93%) | 1 (6%) |
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