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Snake venom is a complex mixture of proteins and peptides, and a …


Biology Articles » Zoology » Herpetology » Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys » Figures

Figures
- Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys

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Figure 1   Analysis of Agki proteome by shotgun-LC/MS approach

(A) Elution profile of digestive peptides from Agki venom from HPLC monitored at 214 nm. (B) Peptide base peak ion chromatogram of digestive peptides from Agki venom. (C) An MS survey scan at time 82.13 min during LC-MS analysis. The parent ion 567.11 was selected further MS/MS analysis. (D) The MS/MS spectra for parent ion 567.11. The amino acid sequence, FVELVLVADK, was confirmed by analysing b- and y-ions derived from the peptide ion.

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Figure 2   Separation of the venomous proteins by SDS/15% PAGE with Coomassie Brilliant Blue staining

Lane M, protein ladder; lane A, Naja venomous proteins; lane B, Agki venomous proteins. MM values are given in kDa.

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Figure 3   Separation of the venomous proteins by GF (Sephadex G-50)

(A) Naja. (B) Agki. Upper panels, the elution profiles of snake venomous proteins monitored at 260 nm and 280 nm. Lower panels, the eluted fractions analysed by SDS/15% PAGE with Coomassie Brilliant Blue staining. MM values are given in kDa. mAU, milli-absorbance units.

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Figure 4   Separation of the venomous proteins by 2DE approach

(A) The Naja venomous proteins collected from G-50 GF with MM more than 10 kDa loaded on to SDS/10% PAGE. (B) The diluted Agki venomous proteins loaded on to SDS/12% PAGE. The pH gradient ranged from 3 to 10 and the gels were stained by Coomassie Brilliant Blue. MM values are given in kDa.

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Scheme 1   Schematic overview of combination strategy for analysing the proteome of snake venom

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