Login

Join for Free!
118908 members
table of contents table of contents

Biomonitoring programs that use mussels to assess the water quality around the …


Biology Articles » Hydrobiology » Proteomic Analysis of Mussels Exposed to Fresh and Weathered Prestige’s Oil

Abstract
- Proteomic Analysis of Mussels Exposed to Fresh and Weathered Prestige’s Oil

Proteomic Analysis of Mussels Exposed to Fresh and Weathered Prestige’s Oil

Itxaso Apraiz1,#, Giulia Leoni1,*,§, David Lindenstrand2, Jan-Olov Persson2, and Susana Cristobal1,#

1Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden
2Department of Mathematics and Statistics, Stockholm University, SE-106 91 Stockholm, Sweden
*Corresponding author: Susana Cristobal, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden
#These authors contributed equally to this work
§Present address: Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, United Kingdom

J Proteomics Bioinform 2: 255-261. doi:10.4172/jpb.1000084. [Open Access Article distributed under the terms of the Creative Commons Attribution License]

Abstract

Biomonitoring programs that use mussels to assess the water quality around the world could benefit from the use of proteomics techniques. These could be applied to obtain protein expression signatures of exposure to pollution that could be further used for prediction purposes. This would require that a combination of univariate and multivariate statistical analyses of proteomics data were utilized to obtain robust models. We show an application of this approach on mussels exposed to fresh fuel, and weathered fuel in a laboratory experiment that tried to mimic the effects of the Prestige’s oil spill. By the combination of those statistical analyses, a set of protein spots were selected that could be used to classify mussels exposed to the two types of fuel oil. As an example of the possibilities that this approach could offer to biomonitoring programs, mussels were collected from ten sampling stations along the NW and NE coasts of the Iberian Peninsula, and their protein expression patterns monitored.

 

Keywords

Prestige oil spill; Fresh fuel; Weathered fuel; Mussels; Two-dimensional electrophoresis (2-DE); Two-way ANOVA; False discovery rate (FDR); Principal components analysis (PCA)

Abbreviations

2-DE: two-dimensional electrophoresis, PES: protein expression signature, ANOVA: analysis of variance, FDR: false discovery rate, PCA: principal component analysis

rating: 0.00 from 0 votes | updated on: 12 Jul 2009 | views: 6440 |

Rate article:







excellent!bad…