The present data show that the splicing factor SF3b1 is a …

• Abstract
• Background
• Results
• Discussion
• Conclusion
• Methods
• List of Abbreviations
• References
• Figures

1. Hastings ML, Krainer AR: Pre-mRNA splicing in the new millennium.

   Curr Opin Cell Biol 2001, 13:302-309.

2. Misteli T: RNA splicing: what has phosphorylation got to do with it?

   Curr Biol 1999, 9:R198-R200.

3. Wang C, Chua K, Seghezzi W, Lees E, Gozani O, Reed R: Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.

   Genes Dev 1998, 12:1409-1414.

4. Manley JL, Tacke R: SR proteins and splicing control.

   Genes Dev 1996, 10:1569-1579.

5. Xiao SH, Manley JL: Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2.

   EMBO J 1998, 17:6359-6367.

6. Caceres JF, Screaton GR, Krainer AR: A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm.

   Genes Dev 1998, 12:55-66.

7. Du C, McGuffin ME, Dauwalder B, Rabinow L, Mattox W: Protein phosphorylation plays an essential role in the regulation of alternative splicing and sex determination in Drosophila.

   Mol Cell 1998, 2:741-750.

8. Duncan PI, Stojdl DF, Marius RM, Bell JC: In vivo regulation of alternative pre-mRNA splicing by the Clk1 protein kinase.

   Mol Cell Biol 1997, 17:5996-6001.

9. Misteli T, Spector DL: Serine/threonine phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors.

   Mol Biol Cell 1996, 7:1559-1572.

10. Misteli T, Caceres JF, Clement JQ, Krainer AR, Wilkinson MF, Spector DL: Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo.

    J Cell Biol 1998, 143:297-307.

11. Seghezzi W, Chua K, Shanahan F, Gozani O, Reed R, Lees E: Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells.

    Mol Cell Biol 1998, 18:4526-4536.

12. Gozani O, Potashkin J, Reed R: A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.

    Mol Cell Biol 1998, 18:4752-4760.

13. de Graaf K, Hekerman P, Spelten O, Herrmann A, Packman LC, Büssow K, Müller-Newen G, Becker W: Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors.

    J Biol Chem 2004, 279:4612-4624.

14. Alvarez M, Estivill X, de la Luna S: DYRK1A accumulates in splicing speckles through a novel targeting signal and induces speckle disassembly.

    J Cell Sci 2003, 116:3099-1107.

15. Himpel S, Tegge W, Frank R, Leder S, Joost HG, Becker W: Specificity determinants of substrate recognition by the protein kinase DYRK1A.

    J Biol Chem 2000, 275:2431-2438.

16. Himpel S, Panzer P, Eirmbter K, Czajkowska H, Sayed M, Packman LC, Blundell T, Kentrup H, Grötzinger J, Joost HG, Becker W: Identification of the autophosphorylation sites and characterization of their effects in the protein kinase DYRK1A.

    Biochem J 2001, 359:497-505.

17. Duncan PI, Stojdl DF, Marius RM, Scheit KH, Bell JC: The Clk2 and Clk3 dual-specificity protein kinases regulate the intranuclear distribution of SR proteins and influence pre-mRNA splicing.

    Exp Cell Res 1998, 241:300-308.

18. Wiechmann S, Czajkowska H, de Graaf K, Grötzinger J, Joost HG, Becker W: Unusual function of the activation loop in the protein kinase DYRK1A.

    Biochem Biophys Res Commun 2003, 302:403-408.

19. Woods YL, Rena G, Morrice N, Barthel A, Becker W, Guo S, Unterman TG, Cohen P: The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site.

    Biochem J 2001, 355:597-607.

20. Leder S, Weber Y, Altafaj X, Estivill X, Joost HG, Becker W: Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases.

    Biochem Biophys Res Commun 1999, 254:474-479.

21. Hofmann TG, Mincheva A, Lichter P, Dröge W, Schmitz ML: Human homeodomain-interacting protein kinase-2 (HIPK2) is a member of the DYRK family of protein kinases and maps to chromosome 7q32-q34.

    Biochimie 2000, 82:1123-1127.

22. Boudrez A, Beullens M, Waelkens E, Stalmans W, Bollen M: Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1.

    J Biol Chem 2002, 277:31834-31841.

23. Songyang Z, Blechner S, Hoagland N, Hoekstra MF, Piwnica-Worms H, Cantley LC: Use of an oriented peptide library to determine the optimal substrates of protein kinases.

    Curr Biol 1994, 4:973-982.

24. Becker W, Weber Y, Wetzel K, Eirmbter K, Tejedor FJ, Joost HG: Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases.

    J Biol Chem 1998, 273:25893-25902.

25. Fotaki V, Dierssen M, Alcantara S, Martinez S, Marti E, Casas C, Visa J, Soriano E, Estivill X, Arbones ML: Dyrk1A haploinsufficiency affects viability and causes developmental delay and abnormal brain morphology in mice.

    Mol Cell Biol 2002, 22:6636-6647.

26. Campbell LE, Proud CG: Differing substrate specificities of members of the DYRK family of arginine-directed protein kinases.

    FEBS Lett 2002, 510:31-36.

27. Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC: A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.

    EMBO J 2001, 20:4536-4546.

28. Golas MM, Sander B, Will CL, Lührmann R, Stark H: Molecular architecture of the multiprotein splicing factor SF3b.

    Science 2003, 300:980-984.

29. Hofmann TG, Möller A, Sirma H, Zentgraf H, Taya Y, Dröge W, Will H, Schmitz ML: Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2.

    Nat Cell Biol 2002, 4:1-10.

30. Sarcevic B, Lilischkis R, Sutherland RL: Differential phosphorylation of T-47D human breast cancer cell substrates by D1-, D3-, E-, and A-type cyclin-CDK complexes.

    J Biol Chem 1997, 272:33327-33337.

31. Brummelkamp TR, Bernards R, Agami R: A system for stable expression of short interfering RNAs in mammalian cells.

    Science 2002, 296:550-553.

32. Lüscher B, Brizuela L, Beach D, Eisenman RN: A role for the p34cdc2 kinase and phosphatases in the regulation of phosphorylation and disassembly of lamin B2 during the cell cycle.

    EMBO J 1991, 10:865-875.