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The present data show that the splicing factor SF3b1 is a …
Home » Biology Articles » Biochemistry » Protein Biochemistry » The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivo phosphorylation site » List of Abbreviations
List of Abbreviations
- The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivo phosphorylation site
The abbreviations used are: CDK, cyclin-dependent kinase; GFP, green fluorescent protein; GST, glutathione S-transferase; pTP, phosphothreonine-proline, shRNA, small hairpin RNA
KdG carried out most of the experiments and drafted the manuscript. HC performed the experiments shown in Figs. 7 and 8. SR devised conditions for the phosphopeptide mapping. LCP performed the mass spectrometry analysis. RL prepared cyclin E/CDK and established the assay conditions. BL participated in the design of the fingerprinting experiments and final editing of the manuscript. WB conceived of and planned this study and edited the manuscript. All authors read and approved the final manuscript.
We thank Lienhardt Schmitz (Department of Chemistry and Biochemistry, University of Bern, Switzerland) for the gift of the HIPK2 expression plasmid. This work was supported by grants from the Deutsche Forschungsgemeinschaft to WB (Be 1967/1–4) and BL (SFB542 B8).
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