Figure 1: Testing of reagents. Several reagents were tested for transfecting a GFP plasmid in primary hippocampal neurons at 14 d.i.v. GFP positive neurons were checked by using a fluorescent microscope after one and five days. All data were normalized on the Lipofectamine 2000 data.
Figure 2: Hippocampal neurons transfected with Lipofectamine 2000. Transfection of hippocampal neurons at 21 d.i.v. by using Lipofectamine 2000 reagent in association with CombiMag reagent. Neurons were observed 5 days after the transfection experiment.
Figure 3: Hippocampal neurons transfected with NeuroMag. Transfection of Hippocampal neurons at 21 d.i.v. by using NeuroMag reagent. Neurons were observed 5 days post transfection.
Figure 4: Determination of the transfected neuron percentage. All neurons were stained by using a MAP-2 antiboby, 5 days after a GFP plasmid transfection. Positive and negative GFP neurons were counted under a fluorescent microscope.
Figure 5: GFP-NRB2 transfection by using Magnetofection. Transfection was performed by using a GFP-NRB2 plasmid. Localization of the recombinant protein was visualized 5 days after transfection. An immunostaining with a MAP-2 antibody was performed to discriminate neurons from glial cells.