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Biology Articles » Agriculture » Possibilities for the Use of Oil Contaminated Solids for Agricultural Purposes » Materials and Methods

Materials and Methods
- Possibilities for the Use of Oil Contaminated Solids for Agricultural Purposes

For the purpose of identifying potential changes in: the chemical composition of soil, mineral and total oil content; polycyclic aromatic hydrocarbon (PAH) content, and heavy metals, yield achieved, and changes in the plant material, in the greenhouse of the Department for General Agronomy of the Faculty of Agriculture in autumn 2003 a plant growth experiment was set up with four repetitions and the following treatments:

1. Check treatment (clean soil not aff ected by oil well operations), soil taken in the vicinity of the Števkovica – Beničanci (oil-hole Števkovica-4) pipeline rupture location (Muvrin and Benčić, 1992; Kisić et al., 2003; Kisić et al., 2005),

2. Completely contaminated soil – soil taken from the contaminated soil disposal site at the Števkovica 4 central landfi ll,

3. 1/2 clean soil + 1/2 contaminated soil,

4. 2/3 clean soil + 1/3 contaminated soil,

5. 3/4 clean soil + 1/4 contaminated soil,

6. Soil delivered to the pipeline rupture location,

7. 2/3 clean soil + 1/3 crude “fresh” oil,

8. 3/4 clean soil + 1/4 crude “fresh” oil.

The experiment was set up in pots with 4 repetitions, and the experimental area (pot) is 0.05 m2. During crops growth usual agrotechnical measures (chemical treatment and mineral fertilization) were applied. Fundamental chemical analyses of the soil (soil reaction, organic matter content, available phosphorus and potassium, and mineral or organic oil content) were made twice a year: prior to setting up the experiment and aft er the “harvest”.

The main purpose of the research is to identify possibility for growing crops in soils contaminated by hydrocarbons (Van-Camp et al., 2004; ISO 10381, 2005), and the eff ect the said contamination might have on crop yield by determining:

1. The influence of varying quantity of contaminated soil on the crop emergence time, establishment and yield achievment.

2. Changes in chemical composition of the soil: soil reaction, plant available phosphorus and potassium, and organic matter content.


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