The functional analysis database offers information on protocols for plant organelle research. Like the organellome database, the functional analysis database is fully searchable. The Web interface provides 10 categories: biochemical assays, computer analysis, detection and analysis of nucleic acids, detection and analysis of proteins, gene expression, histology, genome mapping, tissue and organelle isolation, plant growth and maintenance methods and visualization and observation of organelles (upper part in Figure 5A). Users can view protocols that are classified in each category by clicking the category name on the right of each icon. In addition, the Web interface of the functional analysis database contains a ‘Search’ field providing three search parameters: the species name, category title and keywords (lower part in Figure 5A). Leaving the input boxes blank retrieves all entries. Upon requests from submitters and users, we will include additional categories.
Protocols in each category are presented as a table with annotations of title, link to PDF file, publication and name and affiliation of a contact person (Figure 5B). Publication information provides access to PubMed in NCBI (http://www.ncbi.nlm.nih.gov/). When a file containing a detailed method is provided from a submitter, users can download it as a PDF file.