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Figure 1. Different patterns of actin filaments visualized with GFP-fimbrin over the course of the cell cycle in suspension-cultured tobacco cells. Five images were drawn from the organellome database and arranged. Each image shows a different stage of the cell cycle. (A) interphase, (B) prophase, (C) metaphase, (D) telophase and (E) S phase.
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Figure 2. The positions of chloroplasts in response to changing light conditions. Six images were drawn from the organellome database and arranged. Images near the top (A–C) and the bottom (D–F) of mesophyll cells are shown. (A and D) dark condition, (B and E) weak-light condition, (C and F) strong-light condition.
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Figure 3. Examples of submitted images with multiple-staining information. Each image was downloaded from the organellome database and arranged after trimming and changing sizes for consistency. (A) A merged image of the protoplast from Arabidopsis leaf cells. Green and red signals show peroxisomes and mitochondria, respectively. (B) A merged image of cultured Arabidopsis cells. Cells expressing the fusion gene GFP-SYP21 (At5g16830) were treated with FM4-64. Green and red signals represent GFP-SYP21 in the prevacuolar compartment and FM4-64 on the tonoplast, respectively. (C) A merged image of a longitudinal section of the elongation zone of the inflorescence stem from Arabidopsis. Green signals show tonoplasts visualized with GFP-gamma TIP, and red signals indicate the autofluorescence from chloroplasts. (D–G) Suspension-cultured tobacco cells expressing GFP-fimbrin were treated with FM4-64. (D) Difference interference contrast, (E) GFP-fimbrin labeling actin filaments, (F) FM4-64 labeling the tonoplast, (G) a merged image of (D–F). (H) ER and ER-derived vesicles, ER bodies, are visualized with a fusion protein of GFP and an ER-retention signal.
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Figure 4. The organellome database graphical user interface. (A) A search window provides five parameters for queries. Users can select an organelle name from the pull-down menu and/or enter text in the text boxes for other parameters. (B) A result window shows a table containing thumbnail images of visualized organelles as well as annotations. Clicking on the image in the left column gives access to the detailed information page. (C) Detailed information on At3g33650 is shown as a representative data page.
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Figure 5. The graphical user interface for the functional analysis database and external links. (A) In the functional analysis database, protocols are classified into 10 categories. Users can show protocols in each category by clicking on the category title. In addition, users can search for data using the plant species name, category title and/or keywords in the search field on the bottom. (B) Protocols in each category are displayed as a table including links to PDF files and related references. (C) On the external links page, users can change the category by clicking on the tabs at the top.
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