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A capable expression vector is mainly characterized by its production efficiency, stability …


Biology Articles » Biotechnology » Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations » Conclusion

Conclusion
- Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

The initial plasmid pJOE4056.2, which already bears a well-working translation initiation signal of T7 gene 10 and the transcription terminator from the rrnB operon, has been mainly improved by insertion of a stem loop and by deletion of one cer site. The resultant plasmid pWA21 therefore seems to perform best for eGFP expression in our system. Further changes might be critical as they carry the risk of worsen it. Hence, it seems as there hardly will be any room for further improvements.

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