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Biology Articles » Biotechnology » Red Biotechnology » Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail » Table 1
Table 1
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Comparison of the discussed four different enrichment approaches |
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| DGC |
RS+DGC |
DGC+MCS |
DGC+FACS |
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| Purity |
ca. 70% a |
ca. 93% a |
> 98% d |
90 – 95% f |
| CD5+/CD19+ |
CD5+/CD19+ |
> 93% e |
CD5+/CD19+ |
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| CD19+ |
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| 86% (± 14%) b |
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| CD19+ |
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| 90% (± 7%) c |
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| CD5+/CD19+/CD23+ |
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| Time of procedure |
< 60 mins. |
< 90 mins. |
> 120 mins (increasing with sample volume) |
> 120 mins (increasing with sample volume) |
| Selection method |
negative |
negative |
positive |
positive |
| Limitations |
highly depending on WBC count |
dependent on WBC count when < 20 × 106 cells/ml PB |
long process time and expensive |
long process time and very expensive |
| cost |
low |
medium |
medium-high |
high |
| Required Infra-structure |
centrifuge |
centrifuge |
centrifuge + MCS Sorter |
centrifuge + FACS sorter |
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DGC: density gradient centrifugation, RS+DGC: RosetteSep incubation prior to DGC, DGC+MCS: DGC and subsequent magnetic cell sorting, DGC+FACS: DGC and subsequent fluorescent activated cell sorting. a average purity experienced in our study b purity reported by Haslinger et al., 2004 c purity reported by Falt et al., 2005 d purity reported by Rosenwald et al., 2004 and Wiestner et al., 2003 e Huttman et al., 2006 f purity reported by Stankovic et al., 2004 |
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