Login

Join for Free!
118911 members
table of contents table of contents

High purity of tumour samples is a necessity for accurate genetic and …


Biology Articles » Biotechnology » Red Biotechnology » Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail » Table 1

Table 1
- Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail

Comparison of the discussed four different enrichment approaches

  DGC
RS+DGC
DGC+MCS
DGC+FACS

Purity
ca. 70% a
ca. 93% a
> 98% d
90 – 95% f
  CD5+/CD19+
CD5+/CD19+
> 93% e
CD5+/CD19+
      CD19+
 
  86% (± 14%) b
     
  CD19+
     
  90% (± 7%) c
     
  CD5+/CD19+/CD23+
     
Time of procedure
< 60 mins.
< 90 mins.
> 120 mins (increasing with sample volume)
> 120 mins (increasing with sample volume)
Selection method
negative
negative
positive
positive
Limitations
highly depending on WBC count
dependent on WBC count when < 20 × 106 cells/ml PB
long process time and expensive
long process time and very expensive
cost
low
medium
medium-high
high
Required Infra-structure
centrifuge
centrifuge
centrifuge + MCS Sorter
centrifuge + FACS sorter

DGC: density gradient centrifugation, RS+DGC: RosetteSep incubation prior to DGC, DGC+MCS: DGC and subsequent magnetic cell sorting, DGC+FACS: DGC and subsequent fluorescent activated cell sorting.

a average purity experienced in our study

b purity reported by Haslinger et al., 2004

c purity reported by Falt et al., 2005

d purity reported by Rosenwald et al., 2004 and Wiestner et al., 2003

e Huttman et al., 2006

f purity reported by Stankovic et al., 2004


rating: 2.00 from 1 votes | updated on: 29 Nov 2008 | views: 4980 |

Rate article:







excellent!bad…