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High purity of tumour samples is a necessity for accurate genetic and …


Biology Articles » Biotechnology » Red Biotechnology » Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail » Miscellaneous

Miscellaneous
- Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail

Abbreviations

CLL Chronic Lymphocytic Leukaemia

DGC density gradient centrifugation

FACS fluorescent-activated cell sorting

MCS magnetic cell sorting

PB peripheral blood

PBS Dulbecco's phosphate buffered saline

PCR polymerase chain reaction

RIN RNA Integrity Number

RS RosetteSep™

RS+DGC addition of RosetteSep B-cell enrichment cocktail prior to density gradient centrifugation

RT-PCR reverse transcription polymerase chain reaction

WBC white blood cell

Authors' contributions

SE adapted the methodology, carried out the laboratory work, participated in experimental design, and drafted the manuscript. DC participated in the experimental design and supplied clinical specimens. DW participated in the experimental design and supplied clinical specimens. PG assisted with the immunophenotyping. JFS supplied clinical specimens. AD participated in experimental design, supervised the laboratory work and brought the manuscript to its final form. All authors read and approved the final manuscript.

Acknowledgements

We thank Nancy Messino for assisting with the immunophenotyping and Sophia Halley, Ida Candiloro, Angela Tan, Lasse Kristensen, Michael Krypuy and Chelsee Hewitt for critical reading of the manuscript. Funding for this study was from grants from the CLL Global Research Foundation to AD and JFS and Reece Australia to DW and AD. SE also acknowledges his academic supervisors at the Heinrich Heine University, Prof. Dr. Heinz Mehlhorn and Prof. Dr. Christopher Poremba.


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