High purity of tumour samples is a necessity for accurate genetic and …

• Abstract
• Background
• Results and Discussion
• Conclusion
• Methods
• Miscellaneous
• References
• Table 1
• Figure

This study shows that negative selection using a bifunctional rosette-based antibody cocktail is an effective method to isolate CLL cells of high purity, especially in samples with a WBC count above 20 × 10⁶ cells/ml. The short purification time, the independence from expensive and time consuming procedures, such as FACS and MCS (see Table 1), and the flexible adjustment of cell yields makes RS+DGC an attractive purification method for a wide spectrum of downstream applications, particularly expression analysis utilising microarrays, in which a CLL purity of >90% is desirable.