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High purity of tumour samples is a necessity for accurate genetic and …


Biology Articles » Biotechnology » Red Biotechnology » Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail » Conclusion

Conclusion
- Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail

This study shows that negative selection using a bifunctional rosette-based antibody cocktail is an effective method to isolate CLL cells of high purity, especially in samples with a WBC count above 20 × 106 cells/ml. The short purification time, the independence from expensive and time consuming procedures, such as FACS and MCS (see Table 1), and the flexible adjustment of cell yields makes RS+DGC an attractive purification method for a wide spectrum of downstream applications, particularly expression analysis utilising microarrays, in which a CLL purity of >90% is desirable.

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