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The identification of cells that form tubular structures in response to stress.


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Biology Articles » Zoology » Mammalogy » Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism » Table 1

Table 1
- Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism

Percent radioactivity recovery in cellular fractions from (1-14C) glucose labeled MCF7 cells

  Lipid-phase
 Inter-phase
 TCA
 Ethanol
 Aqueous
Zero Incubation
 0.8
 5
 6
 0.02
 88
Full Incubation
 0.3
 6
 10
 0.07
 85
Transform ation
 0.5 ± 0.2
 12 ± 3
 21 ± 3
 0.4 ± 0.2
 66 ± 6

The lipid-phase is the bottom fraction from the chloroform:methanol extraction of the cells. The inter-phase contains cell debris and proteins, and the TCA fraction contains proteins from precipitation of the aqueous fraction. The ethanol fraction contains carbohydrates from the remaining aqueous fraction in 5 volumes of ice-cold ethanol incubated at -20°C over night. The remaining water-soluble fraction contains unused 14C-glucose.


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