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The identification of cells that form tubular structures in response to stress.


Biology Articles » Zoology » Mammalogy » Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism » Table 1

Table 1
- Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism

Percent radioactivity recovery in cellular fractions from (1-14C) glucose labeled MCF7 cells

  Lipid-phase
Inter-phase
TCA
Ethanol
Aqueous

Zero Incubation
0.8
5
6
0.02
88
Full Incubation
0.3
6
10
0.07
85
Transform ation
0.5 ± 0.2
12 ± 3
21 ± 3
0.4 ± 0.2
66 ± 6

The lipid-phase is the bottom fraction from the chloroform:methanol extraction of the cells. The inter-phase contains cell debris and proteins, and the TCA fraction contains proteins from precipitation of the aqueous fraction. The ethanol fraction contains carbohydrates from the remaining aqueous fraction in 5 volumes of ice-cold ethanol incubated at -20°C over night. The remaining water-soluble fraction contains unused 14C-glucose.


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