Mouse tissue lysis for genotyping
This is a quick protocol for mouse tail and tissue lysis with proteinase K. It is commonly used to prepare templates for genotyping. Other protocols included detergents in the lysis buffer, but we found this simple protocol to work well with less hands-on time.
tissue lysis to release DNA
per tail or tissue chunk add (tissue degradation is sped up if tissue/tail is cut into smaller bits):
proteinase K inactivation
storage before PCR
rating: 0.00 from 0 votes | updated on: 21 Aug 2007 | views: 2011 |