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Figure 1 One active cryoprobe in vital metaphysial bone (proximal tibia) Fig. 1a: Experimental set up using one cryoprobe on the tibial metaphysis Two active cryoprobes in vital diaphysial bone (femoral diaphysis) Fig. 1b: Experimental set up simultaneously using two cryoprobes

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Figure 2 Gelatin – 1 cryoprobe vertical measurement
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Figure 3 Gelatin – 1 cryoprobe horizontal measurement
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Figure 4 Initial melting of the ice crystals at the equator of both edges
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Figure 5 Gelatin – 2 cryoprobes horizontal measurement
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Figure 6 In-vivo metaphyseal bone – 1 cryoprobe
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Figure 7 In-vivo metadiaphyseal bone – 2 cryoprobes

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Figure 8 Total cortical necrosis with empty osteocyt lacuna and without detectable nucleous staining of the tibia

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Figure 9 In contrast a healthy areal of the same sheep and the same tibia with stained nucleus of the osteocyts
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