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This study describes the microscopic anatomy of the reproductive systems of the …


Biology Articles » Anatomy & Physiology » Anatomy, Animal » Microscopic Anatomy of the Reproductive System in Two Sympatric Species of Fissurella Bruguiére, 1789 (Mollusca: Vetigastropoda) » Material and Method

Material and Method
- Microscopic Anatomy of the Reproductive System in Two Sympatric Species of Fissurella Bruguiére, 1789 (Mollusca: Vetigastropoda)

Twelve individuals of each sex of F. ¡atimarginata andi cumingi were collected from the rocky subtidal zone at Montemar, V Region, Chile (32° 56' S; 71° 32' W). Individuals sampled were assigned to each taxa following the features of the shell and external anatomy proposed by McLean (1984) and Oliva & Castilla (1992). For histological analysis, dissected specimens were pre-fixed for 2 h in a formalin-alcohol-acetic acid solution (FAA). Tissue pieces containing the reproductive system was sectioned transversally from anterior to posterior to give pieces of approximately 10 mm in thickness, and then post-fixed 24 h in FAA. The pieces were rinsed and dehydrated in 95% ethanol, and processed by routine histological techniques until to get permanent slides (Valderrama et al., 2004). YorF. latimarginata, serial sections (10-12 Lim) were obtained transversally to the anteroposterior axis, sagitally to the laterolateral axis and longitudinally to the dorsoventral axis of the individuals. YorF. cumingi, sections transversal to the anteroposterior axis were made. The sections were stained using a trichrome method for topographic coloration (Valderrama et al.) and analyzed in a bright field microscope.

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