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Xylella fastidiosa (Xf) is a xylem-limited bacterium that lives as a harmless …


Biology Articles » Botany » Plant Pathology » Leaf scorch symptoms are not correlated with bacterial populations during Pierce's disease » Figures

Figures
- Leaf scorch symptoms are not correlated with bacterial populations during Pierce's disease

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Fig. 1. (A) ClustalW multiple nucleotide sequence alignment of the target portion of EFTu of two Xylella fastidiosa strains, 9a5c and Temecula, and three Xanthomas species. Base pair mismatches are shaded. (B) Agarose gel electrophoresis of PCR products resulting from a reaction with the forward and reverse primers and total genomic DNA from the labelled species. The expected size of product from Xf is indicated by an arrow.

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Fig. 2. Comparison of standard curves produced with various DNA isolation methodologies. Standard curves produced from mixed plant leaf/Xf DNA isolated using a standard CTAB extraction (open circles), Qiagen Plant Kit (closed triangles), or Epicentre Masterpure Plant Kit (open triangles) compared with that produced from Xf genomic DNA isolated from culture (closed circles). Error bars represent ±1 standard deviation.

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Fig. 3. (A–R) Photographs of sampled greenhouse-grown Chardonnay leaves. The leaf punches sampled are to scale (shaded), and Xf populations in those punches are given as log10 cells g–1 fresh weight tissue or none detected (nd). Leaves are arranged in order of increasing symptom severity from those with no symptoms (symptom severity rating of 1) to those exhibiting severe symptoms (symptom severity rating of 5). Two of the leaves analysed (P and Q) were those originally inoculated with Xf (indicated with red dots). Scale bars=1.5 cm.

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Fig. 4. (A) Average Xf populations (log10 cells g–1 of fresh weight tissue) in leaves with various symptom severities. Error bars represent standard error. (B) Relationship between average Xf populations and the date on which the samples were taken. The difference is significant (P

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Fig. 5. Xf populations in field-grown Chardonnay leaves. (A, B) Representative photographs of plants exhibiting severe (A) or moderate (B) symptoms of Xf infection, including green islands and matchsticks (white arrows). (C, D) Symptom severity and bacterial population for leaves from plants exhibiting severe (C) or more moderate (D) symptoms. (E) Percentage of leaves with various populations of Xf for samples from plants exhibiting severe (grey bars) or moderate (black bars) symptoms.

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