Quantifying 2D kinetics and forced dissociations of receptor-ligand interactions in cell adhesions is crucial to further the understandings in immune responses. A probabilistic model of small system is developed to predict 2D kinetic rates and binding affinities, while a chemical- mechanical coupling model is introduced to analyze forced regulation of receptor-ligand interactions. The state-of-the-art techniques including micropipette aspiration and atomic force microscopy have been used to measure the receptor-ligand binding kinetics and regulation of applied forces. Structural variation, surface environment, and membrane microtopology and stiffness affect the kinetic rates and affinities. Applied forces regulate the bond strength and lifetime in multiple phases.
This work was supported by NSFC grants 10332060 and 30225027, and CAS grants KJCX2-SW-L06 and 2005-1-16.