Primordial germ cells (PGCs) are the embryonic precursors of the sperm and …

• Abstract
• Background
• Results
• Discussion
• Conclusion
• Methods
• Miscellaneous
• References
• Figures

Abbreviations

BSA: bovine serum albumin; DHCR7: 3b-hydroxysterol-Δ7 reductase; E9.5: embryonic day 9.5; GFP: green fluorescent protein; HH: hedgehog; HMGCR: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; KITL: kit ligand; NR5A1: steroidogenic factor 1; PARP: poly(ADP-ribose) polymerase 1; PBS:phosphate buffered saline; PGC: primordial germ cell; RT-PCR: real time polymerase chain reaction; SC5D: lathosterol 5-desaturase; SCARB1: scavenger receptor class b member 1; SDF1: stromal derived factor 1; TOF-SIMS: time of flight secondary ion mass spectrometry; UGR:urogenital ridge.

Authors' contributions

JD performed and quantified the statin treatments, filipin staining and PARP staining. The initial draft of the results section was prepared as part of her undergraduate honors thesis. DJ performed the bioprobe measurements. The current response data was analyzed by DJ and JB. MK, AE and NW performed TOF-SIMS analysis. JN checked plugs and maintained the Oct4ΔPE:GFP mouse colony. BD prepared the genital ridge and midline cDNA samples for real time PCR. EM and FP isolated and genotyped the Dhcr7 and Sc5d embryos originally generated by FP. KM performed the SSEA1 staining, rescue experiments and time lapse experiments and drafted the manuscript.

Acknowledgements

We acknowledge Patty Conrad for microscopy assistance. Funding support for the Leica AOBS confocal multi-user facility was supplied by a grant from NIH-NCRR (RR-017980-01). We thank Joe Nadeau, Jenny Liang and Helen Salz for critical reading of the manuscript. The MC-480/SSEA1 antibody developed by David Solter was obtained for the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242. Financial support for this project was supplied by Case Western. This work was also funded in part by the intramural research of the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD).