BSA: bovine serum albumin; DHCR7: 3b-hydroxysterol-Δ7
reductase; E9.5: embryonic day 9.5; GFP: green fluorescent protein; HH:
hedgehog; HMGCR: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; KITL:
kit ligand; NR5A1: steroidogenic factor 1; PARP: poly(ADP-ribose)
polymerase 1; PBS:phosphate buffered saline; PGC: primordial germ cell;
RT-PCR: real time polymerase chain reaction; SC5D: lathosterol
5-desaturase; SCARB1: scavenger receptor class b member 1; SDF1:
stromal derived factor 1; TOF-SIMS: time of flight secondary ion mass
spectrometry; UGR:urogenital ridge.
JD performed and quantified the statin treatments, filipin staining
and PARP staining. The initial draft of the results section was
prepared as part of her undergraduate honors thesis. DJ performed the
bioprobe measurements. The current response data was analyzed by DJ and
JB. MK, AE and NW performed TOF-SIMS analysis. JN checked plugs and
maintained the Oct4ΔPE:GFP mouse colony. BD
prepared the genital ridge and midline cDNA samples for real time PCR.
EM and FP isolated and genotyped the Dhcr7 and Sc5d embryos
originally generated by FP. KM performed the SSEA1 staining, rescue
experiments and time lapse experiments and drafted the manuscript.
We acknowledge Patty Conrad for microscopy assistance. Funding
support for the Leica AOBS confocal multi-user facility was supplied by
a grant from NIH-NCRR (RR-017980-01). We thank Joe Nadeau, Jenny Liang
and Helen Salz for critical reading of the manuscript. The MC-480/SSEA1
antibody developed by David Solter was obtained for the Developmental
Studies Hybridoma Bank developed under the auspices of the NICHD and
maintained by the University of Iowa, Department of Biological
Sciences, Iowa City, IA 52242. Financial support for this project was
supplied by Case Western. This work was also funded in part by the
intramural research of the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD).