L-Arg, L-Arginine; GFP, Green Fluorescent Protein; NO, Nitric oxide; NOS, nitric oxide synthase; L-NNA, NG- nitro-L-Arginine; NeuroD1, Neurogenic Differentiation 1; PDX-1, Pancreatic Duodenum Homeobox protein-1; MafA, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A
The author(s) declare that they have no competing interests.
LM performed all of the experiments, data quantification and
drafting of the manuscript. SÖ substantially contributed to the
conception and design of the experiments, revised the manuscript
critically for important intellectual content. Both authors read and
approved the final manuscript.
We thank Dr. Graeme Bell (University of Chicago) for the human
insulin cDNA, Dr. Brett Spear for Hepa1-6 cell line, Dr. Bert
Vogelstein for the pAdEasy adenovirus system and Drs. Howard Glauert
and Job Tharappel for the primary hepatocyte isolation protocol. We
also thank Drs. Qingwen Qian, Zheng-Shan Zhang and Wei Ren for the
NeuroD1 and MafA adenoviruses. We thank.Vinobalan Durairaj for help
with statistical analysis and the members of our laboratory for helpful
discussions. This work was supported by NIH/NIDDK grants 5R21DK0644829
and 5R01DK067581 (to S.Ö.), by NIH grant P20RR020171 from the National
Center for Research Resources, and by a Postdoctoral Fellowship from
the American Heart Association, Ohio Valley Affiliate (to L.M.).