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A study on the immunomodulatory role of curcumin in a mouse model …


Biology Articles » Immunobiology » Immune response modulation by curcumin in a latex allergy model » Figures

Figures
- Immune response modulation by curcumin in a latex allergy model

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Figure 1 Total serum IgE, peripheral blood eosinophils, latex specific antibodies, and cytokine responses. A. Total serum IgE in nanogram per ml in controls (Group1); latex sensitized mice (Group 2); and latex sensitized mice treated with curcumin (Group 3). B. Peripheral blood eosinophils in the three groups. C. Serum IgG1,2a,2b, and IgG3 latex specific antibodies. The antibody levels were calculated from the O.D. values of at least five two-fold dilutions and log titers calculated using a computerized program. D. IFN-γ production (ng/ml) by antigen-stimulated spleens cells as measured by ELISA. E. IL-4 production (Pg/ml) by antigen-stimulated spleen as measured by ELISA.

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Figure 2 Relative mRNA expression of CD205, MMP-9, OAT, and TSLP in the lungs of control and experimental mice.

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Figure 3 Expression of costimulatory molecules on cells from the lungs of control, latex-sensitized, and latex-sensitized mice treated with curcumin. Lung cells were pooled from mice in each group and analyzed by flow cytometry. Representative histograms comparing CD80 expression on B cells from the three groups tested are shown in panel A1. CD80 expression is shown as percent positive (% Pos) cells in Panel A2, and as median fluorescence (FL) values in Panel A3. The percentages of positive cells and median fluorescence values are shown for CD86 and OX40L on B cells in Panels B and C, respectively. Similarly, CD80, CD86, and OX40L expression on lung macrophages is shown for the three groups in Panels D, E, and F. Percentages of CD4+ T cells expressing OX40 and the median fluorescence values for OX40 are shown in Panel G.

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Figure 4 Histology of the lungs studied from control and experimental mice. A. Lungs of the control mice stained by Hematoxylin and eosin (H&E) ×40. B. Lung tissues stained with PAS ×40. C. Latex challenged mice, H&E at ×40. D. Latex challenged mice at ×400. E. Latex challenged mice stained with PAS at ×400. F. Latex challenged mice stained with PAS at ×400. G. Lung section from curcumin treated mice (Group 3), H&E at ×40. H. Lung sections from curcumin treated mice but magnification H&E at ×400. I. Lung section from curcumin treated mice stained with PAS at ×40.

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Figure 5 Immunohistochemical staining for IL-4, IL-5, IL-13, and IFN-γ of control mice (Group 1), latex challenged mice (Group 2), and latex challenged treated with curcumin (Group 3). A. IL-4; B. IL-5; C. IL-13; and D. IFN-γ.

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