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These findings contribute to define the growing group of human polyserine proteases …
Biology Articles » Biochemistry » Protein Biochemistry » Identification and characterization of human polyserase-3, a novel protein with tandem serine-protease domains in the same polypeptide chain » Abbreviations
- Identification and characterization of human polyserase-3, a novel protein with tandem serine-protease domains in the same polypeptide chain
The abbreviations used are: AEBSF, 4-(2-aminoethyl)-benzenesulfonyl fluoride; bp, base pair(s); DAPI, 4'-6'-diamidino-2-phenylindole hydrochloride; GST, glutathione S-transferase; PAGE, polyacrylamide gel electrophoresis; PCR, polymerase chain reaction; RACE, rapid amplification of cDNA ends; RT, reverse transcription; SDS, sodium dodecyl sulfate; Spd, serine protease domain; TTSP, type II transmembrane serine proteinase; uPA, urokinase-type plasminogen activator.
S.C. participated in the design of the study and performed the identification and cloning of polyserase-3. He also contributed to the elaboration of the manuscript. J.-R.P. carried out the expression of the recombinant protein in different systems and performed the molecular modelling studies. M.L. collaborated in the molecular cloning studies and performed tissue expression analysis of polyserase-3. V.Q. participated in the purification and enzymatic analysis of the recombinant proteins. A. M.-P. carried out the evolutionary analysis of polyserase-3. C.G. performed studies with the different cell lines. C.L.-O. conceived the study, participated in its design and wrote most sections of the manuscript. All authors read and approved the final manuscript.
This work was supported by grants from Ministerio de Ciencia y Tecnología-Spain and European Union (Cancer Degradome-FP6). The Instituto Universitario de Oncología is supported by Obra Social Cajastur-Asturias and Red de Centros de Cancer-Instituto Carlos III, Spain. S.C. is a "Ramón y Cajal" research fellow. We thank L.M. Sánchez for helpful comments, and M. Fernández and S. Alvarez for excellent technical assistance. We specially thank Paz Serrano her generous support to our research group. This paper is dedicated to the memory of Daniel Vázquez Esteban.
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