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Using transgenic mouse model of diabetes and controls to examine the histological …


Biology Articles » Biochemistry » Enzymology » Hyperglycemia alters enzyme activity and cell number in spinal sensory ganglia » Figures

Figures
- Hyperglycemia alters enzyme activity and cell number in spinal sensory ganglia

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Figure 1 Enzyme histochemical reaction product in the fifth lumbar dorsal root ganglion from control and diabetic mice. (A,B) Cytochrome oxidase reaction product in sections from control (A) and the diabetic (B) mice. Diabetic mice display reduced levels of CO reaction product compared to control. Quantitative analysis revealed a decrease in CO reaction product density in small neuronal somata. (C,D) NADPH-diaphorase reaction product in sections from control (C) and diabetic (D) mice. Diabetic mice display an increase in NADPH-diaphorase reaction product density compared to control. Scale bar in microns.

figure 1

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Figure 2 Enzyme histochemical reaction product in the dorsal horn of the fifth lumbar spinal cord from control and diabetic mice. (A,B) Acid phosphatase reaction product in sections from control (A) and the diabetic (B) mice. Diabetic mice display reduced levels of AP reaction product in medial lamina I and II of the dorsal horn. (C,D) NADPH-diaphorase reaction product in lamina I and II of control (C) and diabetic (D) mice. A reduction in the number of labeled fibers and somata can be observed in the superficial dorsal horn of diabetic animals. Scale bar in microns and dorsal horn is to top in all sections.

figure 2

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